3D Neural Connection and Visceral Pain Receptor Atlas for Genitourinary Tract Dev

描述(由申请人提供)：NIDDK资助了一项计划，旨在全面了解伤害性或疼痛感受器系统如何在小鼠胚胎中发育并在成年后成熟。这一基本认识将为慢性盆腔疼痛和类似健康状况的临床前和临床研究提供基础，这些疾病影响1-6%的女性和1-3%的男性。该项目将捕捉小鼠胚胎中与疼痛相关的神经系统发育的三维图像。我们将使用分子生物学技术将发育中的神经元以一系列可以用共焦显微镜捕捉到的颜色进行可视化。为了以看到单个神经纤维所需的分辨率捕捉胚胎区域的完整图像(约千分之一毫米)，我们将使用英国发明的一种新的介镜显微镜，它可以无损地扫描整个胚胎。由此产生的图像将提供大量的数据和信息，以挖掘细胞的细节。我们将通过基于网络浏览器的查看器将图像数据提供给研究社区和公众，使研究人员能够以全分辨率查看卷的任何部分。这些数据将向公众免费提供，并可下载以供进一步研究。我们将捕捉这些3D图像的时间序列，以首次构建哺乳动物疼痛感受器和感觉系统发展的详细但完整的视图。有关发育中神经的信息将保存在一个数据库中，该数据库链接到NIH/NIDDK资助的生殖器分子解剖学项目(GUDMAP)数据库。

DESCRIPTION (provided by applicant): The NIDDK has funded a program to develop a comprehensive understanding of how nociceptive or pain receptor systems develop in the mouse embryo and mature in the adult. This basic understanding will provide a foundation for pre-clinical and clinical research into chronic pelvic pain and similar health conditions, which affect 1-6% of women and 1-3% of men. This project will capture three-dimensional images of the developing nervous systems relating to pain in the mouse embryo. We will use molecular biology techniques to visualize the developing neurons in a range of colors that can be captured using a confocal microscope. To capture a full image of the embryo region at the resolution needed to see individual nerve fibers (about 1 thousandth of a millimeter) we will use a new Mesoscope microscope invented in the UK, which can scan the whole embryo non-destructively. The resulting images will provide a massive volume of data and information to be mined for cellular detail. We will deliver the image data to the research community and public via web-browser based viewers that allow the researcher to view any part of the volume at full resolution. The data will be freely accessible to the public and can be downloaded for further research. We will capture a time-series of these 3D images to build for the first time a detailed but complete view of the development of the pain receptor and sensory systems in mammals. The information about the developing nerves will be held in a database linked to the NIH/NIDDK funded Genitourinary Molecular Anatomy Project (GUDMAP) database.