PROTEOMICS

项目概述（见说明）：蛋白质组学共享资源为纽约大学的癌症研究人员提供最先进的生物质谱分析，帮助他们识别和表征具有医学重要性的蛋白质。在许多情况下，只有通过直接研究蛋白质，人们才能对细胞和组织在正常和疾病状态（如癌症）下的功能过程有一个有用的理解。共享资源具有在单次分析中识别单个，数百甚至数千种蛋白质的能力，通常在亚微摩尔水平，通过最先进的液相色谱-串联质谱法，然后进行数据库搜索或从头序列测定。在大多数情况下，该设施努力从各种条件下的细胞中获得有关这些蛋白质的绝对和/或相对数量的准确信息，以获得有关蛋白质功能的重要信息。共享资源还具有表征蛋白质翻译后修饰的特殊专业知识，如磷酸化，酰化，糖基化和泛素化。除了其技术专长外，该设施的优势之一是能够在实验的设计和解释方面向客户提供建议，以便获得有用的数据，并从这些数据中获得有意义的信息。依靠尖端技术及其专业和敬业的员工，这些服务能够以具有成本效益的方式进行。事实上，如果没有共享资源的帮助，纽约大学和纽约大学癌症研究所（NYUCI）的研究人员的许多实验是不可能完成的。该资源还建立了临床蛋白质组学核心，用于检测蛋白质和肽生物标志物，用于癌症的早期检测。

PROJECT SUMMARY (See instructions): The Proteomics Shared Resource provides state-of the-art biological mass spectrometry analyses to cancer researchers at NYU to help them identify and characterize proteins of medical importance. In many cases, it is only by studying proteins directly that one can achieve a useful understanding of the processes that underly the functioning of cells and tissues in normal and diseased states such as cancer. The shared resource has the capability to identify single, hundreds or even thousands of proteins in a single analysis, often at subfemtomole levels, by state of the art liquid chromatography-tandem mass spectrometry followed by database searching or de novo sequence determination. In most cases the facility strives to obtain accurate information about the absolute and/or relative quantities of these proteins from cells under various conditions to obtain important information about the functioning of the proteins. The shared resource also has special expertise in characterizing posttranslational modifications of proteins such as phosphorylation, acylation, glycosylation and ubiquitination. In addition to its technical expertise, one of the strengths of the facility is its ability to advise clients in the design and interpretation of experiments so that useful data can be obtained and meaningful information can be had from these data. Reliance on cutting edge technology and its expert and dedicated staff allow these services to be performed in a cost effective manner. Indeed, many experiments by investigators at NYU and the NYU Cancer Institute (NYUCI) could not have been done without the assistance of the Shared Resource. The Resource also has established a Clinical Proteomics Core for the detection of protein and peptide biomarkers for the early detection of cancer.