Metal-titanates as Novel Inhibitors of Cariogenic Biofilms at Tooth-composite Int

描述（由申请人提供）：本研究的目标是开发一类新的抗菌纳米颗粒钛酸盐-金属络合物。已显示出微钛酸盐-金属络合物具有抗微生物活性。目前，还没有关于纳米钛酸盐与细菌的相互作用以及金属离子Au（III）、Pd（II）和Pt（IV）的抗菌性能的数据。本研究的中心假设是，纳米颗粒钛酸盐-金属复合物被纳入牙科粘结树脂中，将抑制致龋细菌在邻近牙齿/复合材料修复界面的生物膜中生长，而不损害树脂-牙齿粘结。这项工作是创新的，因为它利用了一类新的钛酸盐金属络合物作为抗菌剂。此外，生物膜种群动态相对于牙齿/复合材料修复界面将量化非侵入性的单克隆抗体共轭量子点。只有三个关于细菌生物膜的报告，没有一个适用于致龋细菌。所提出的工作的组合预计将允许临床医生解决最弱界面处的复合材料寿命问题。通过探索钛酸盐-金属配合物与细菌生物膜之间的相互作用，我们将填补一类新的钛酸盐-金属配合物抗菌剂的知识空白。 公共卫生相关性：我们的研究将开发一类新的抗菌纳米颗粒钛酸盐-金属配合物的Au（III），Pd（II），和Pt（IV）。我们将把这种钛酸盐-金属复合物加入到牙科粘结树脂中，这将抑制致龋细菌在牙齿/复合材料修复界面附近的生物膜中生长。我们将研究生物膜收集在实验室和从人类受试者与多光子激光显微镜。

DESCRIPTION (provided by applicant): Our goal of this investigation is to develop a new class of antibacterial nanoparticle titanate-metal complexes. Micro titanate-metal complexes have been shown to have antimicrobial activity. At present, there are no data on the interactions of nanoparticle titanate with bacteria and on the antibacterial properties of metal ions of Au(III), Pd(II), and Pt(IV). The central hypothesis of this investigation is that nanoparticle titanate-metal complexes that are incorporated into a dental bonding resin will inhibit cariogenic bacterial growth in a biofilm adjacent to the tooth/composite restoration interface without compromising resin-tooth bonding. The proposed work is innovative, because it capitalizes on a new class of titanate-metal complexes as antibacterial agents. In addition, biofilm population dynamics with respect to tooth/composite restoration interface will be quantified non-invasively by Mab-conjugated quantum dots. There are only three such reports on bacterial biofilms and none applied to cariogenic bacteria. The combination of the work proposed is expected to allow the clinicians to tackle the composite longevity problem at the weakest interface. By exploring the interactions between titanate-metal complexes and bacteria biofilm, we will bridge a major gap in knowledge about a new class of titanate-metal complexes antibacterials. PUBLIC HEALTH RELEVANCE: Our investigation will develop a new class of antibacterial nanoparticle titanate-metal complexes of Au(III), Pd(II), and Pt(IV). We will incorporate this titanate-metal complexes into a dental bonding resin, which will inhibit cariogenic bacterial growth in a biofilm adjacent to the tooth/composite restoration interface. We will study the biofilm collected both in the laboratory and from human subjects with multi-photon laser microscopy.