NIDCR DIR Scientific Cores

NIDCR的ESTA科学核心提供了研究支持校内研究人员进行牙科和颅面疾病的研究。 基因靶向核心（GTC）允许研究人员在小鼠中“添加”或“减去”基因，以开发相关人类疾病的动物模型用于研究，并发现特定基因对宿主生理的影响。 GTC产生嵌合小鼠，导致感兴趣的基因的种系传递。 此外，创建和/或重建新的基因敲除和转基因小鼠。 小鼠胚胎干细胞服务成功完成了基因靶向实验。 GTC还通过冷冻保存小鼠动物模型的胚胎和精子提供了重要的科学支持服务。 这些服务促成了100多份研究和评论出版物（在ZIA项目中报告）。 目前正在测试其他服务，包括小鼠模型的IVF服务和其他小鼠胚胎干细胞系的开发。 联合技术研究核心的形成是为了将几个核心功能置于一个核心主管和一个核心主管之下，包括DNA测序，为NIDCR荧光分析仪领导的研究提供了一千多个反应，流式细胞术和细胞分选，细胞洗脱，激光捕获显微镜和组织学。

The NIDCR DIR Scientific Cores provided research support for intramural investigators performing studies on dental and craniofacial diseases. The Gene Targeting Core (GTC) allows investigators to "add" or "subtract" genes in mice to develop animal models of relevant human diseases for research and to discover the affect of specific genes on host physiology. The GTC generated chimeric mice resulting in germline transmission of the genes of interest. In addition new gene knockout and transgenic mice were created and/or re-established. The murine embryonic stem cell service successfully completed gene targeting experiments. The GTC also provided an important scientific support service by cryopreserving embryos and sperm from murine animal models. These services contributed to over 100 research and review publications (reported in ZIA projects). Testing is underway for additional services, including IVF services for murine models and development of additional murine embryonic stem cell lines. The Combined Technical Research Core was formed to put several core functions under one Core Chief and a Core Director, including DNA Sequencing, which provided over a thousand reactions for NIDCR DIR investigator-led research, Flow Cytometry and Cell Sorting, Cell Elutriation, Laser Capture Microscopy, and Histology.