Analytical Glycotechnology

核心单位作为项目资助资源的作用 核心C概述 分析糖技术核心的目的是提供技术专长， 对分离的唾液酸、N-和O-连接聚糖和GAG进行结构表征所需的仪器 由项目负责人实验室提供的转基因动物细胞或组织。芯会 极大地受益于现有Glycotechnology Core中的仪器、试剂和专业知识 加州大学圣地亚哥分校糖生物学研究和培训中心（GRTC）。许多技术 用于这些分析（鞘糖脂提取、聚糖、唾液酸和唾液酸的分离 衍生物分析，包括NeuSGc，糖基连接分析，包括末端唾液酸连接（a2-3/a2- 3）， 6)使用GC-MS，通过MALDI-TOF MS/ MS-MS和2-AB荧光标记的NP-HPLC进行聚糖分析，GAG 在上一个资助期间，设计了一种新的方法（通过HPLC和ESI-MS进行二糖分析）。的可用性 高品质的仪器，加上多年的糖生物学分析经验， 由糖技术核心资源的成员代表，使我们能够满足分析 项目负责人提出的挑战，并进行必要的分析，以支持他们的研究。

Function of Core Unit as a Resource to the Program Project Grant Core C Overview The purpose of the Analytical Glycotechnology Core is to provide the technical expertise and instrumentation necessary to structurally characterize sialic acids, N- and O-linked glycans and GAGs isolated from cells or tissues from transgenic animals provided by the Project Leader's laboratories. The core will greatly benefit from access to the instruments, reagents, and expertise in the existing Glycotechnology Core Resource of the UCSD Glycobiology Research and Training Center (GRTC). Many of the techniques to be employed for these analyses (glycosphingolipid extraction, isolation of glycans, sialic acid and sialic acid derivative analysis including NeuSGc, glycosyl linkage analysis including terminal sialic acid linkages (a2-3/a2- 6) using GC-MS, glycan profiling by MALDI-TOF MS/ MS-MS and 2-AB fluorescent labeled NP-HPLC, GAG disaccharide analysis by HPLC and ESI-MS) were devised during the previous funding period. The availability of high quality instrumentation, coupled with the many combined years of analytical glycobiology experience represented by the members of the Glycotechnology Core Resource, allow us to meet the analytical challenges proposed by the Project Leaders and to perform the analyses necessary to support their research.