Molecular Screening Shared Resouce

分子筛选共享资源（MSSR）：完成人类基因组序列， 小鼠、斑马鱼和其他一些模式生物的研究，已经初步鉴定了所有编码的 这些物种的蛋白质。化学文库和全基因组文库的增殖 许多物种的抑制性RNA序列使人们有可能设想寻找抑制性RNA序列。 小分子和抑制性RNA的所有基因编码的目标。伴随而来的是 高通量筛选（NTS）技术使人们有可能-通过适当的资源-移动 从概念到实践。UCLA JCCC分子筛选共享资源（MSSR） 成立于2003年，以满足这一令人兴奋的科学目标。MSSR现在有专门的空间， 新的加州纳米系统研究所，小分子文库包含超过70，000种化合物，全基因组 用于酵母的敲除文库和用于鼠和人靶的干扰RNA文库。优秀 学术和技术领导已经建立了一个强大的，可访问的筛选中心，协助双方 开发可靠和强大的酶和细胞培养HTS测定，并促进全基因组 卡位大卫格芬医学院，加州纳米系统研究所（CNSI），加州大学洛杉矶分校 文学与科学学院，几个个别部门和一些私人捐助者加入了 JCCC为设备和图书馆提供资金（远远超过1，000，000美元）并进行“启动” 启动并运行MSSR所需的所有信息共享资源已运行（或当前正在运行）65 屏幕，其中绝大多数是JCCC成员。成员基因调控，信号 转导和治疗学，肿瘤免疫学，癌细胞生物学，造血系统肿瘤， 分子流行病学和致癌作用、胸部肿瘤学和泌尿生殖系统发展项目 肿瘤学项目领域已经利用了这一共享资源。CCSG为此提出的预算要求 CCSG第一年预算的共享资源占估计业务预算的13%; 共享资源也来自CNSI，NIH的辐射防护中心，加州大学洛杉矶分校校长的 生物科学倡议，用户充值和慈善事业。（另请参见第6.2.3节共享 历史上的资源，描述，基本特征）。 代表四个癌症中心项目区的八名癌症中心成员完成了 在本报告所述期间，分子筛选共享资源;许多筛选正在进行中。 这是一个持续的共享资源。

The Molecular Screening Shared Resource (MSSR): Completion of the genome sequences of humans, mice, zebrafish and a number of other model organisms has led to the tentative identification of all encoded proteins in these species. The proliferation both of chemical libraries and of libraries of genome wide inhibitory RNA sequences for a number of species has made it possible to conceive of looking for inhibitory small molecules and inhibitory RNAs for all genetically encoded targets. The concomitant development of High Throughput Screening (NTS) technologies has made it possible - with the right resources - to move this from concept to practice. The UCLA JCCC Molecular Screening Shared Resource (MSSR) was established in 2003 to meet this exciting scientific objective. The MSSR now has dedicated space in the new California NanoSystems Institute, small molecule libraries containing over 70,000 compounds, genomewide knockout libraries for yeast, and interfering RNA libraries for both murine and human targets. Excellent academic and technical leadership has established a robust, accessible screening center that assists both in developing reliable and robust enzymatic and cell culture HTS assays and in facilitating genome-wide screens. The David Geffen School of Medicine, the California NanoSystems Institute (CNSI), the UCLA College of Letters and Science, several individual departments and some private donors have joined the JCCC to provide funding for the equipment and libraries (well over $1,000,000) and operating "start-up" required to get the MSSR up and running. The Shared Resource has run (or is currently running) 65 screens, the great majority of which are for JCCC members. Members of the Gene Regulation, Signal Transduction and Therapeutics, Tumor Immunology, Cancer Cell Biology, Hematopoietic Malignancies, Developing Program in Molecular Epidemiology and Carcinogenesis, Thoracic Oncology and Genitourinary Oncology Program Areas have already utilized this Shared Resource. The CCSG budget request for this Shared Resource for the first year CCSG budget is for 13% of the estimated operating budget; support for the shared resource also comes from the CNSI, an NIH Center for Radioprotectors, the UCLA Chancellor's Biosciences Initiative, user recharges and philanthropy. (Please also see Section 6.2.3 on Shared Resources in the History, Description, Essential Characteristics). Eight Cancer Center members representing four Cancer Center Program Areas completed screens in the Molecular Screening Shared Resource during the reporting period; many screens are ongoing. This is a continuing shared resource.