Application Of Mass Spectrometry To Structural Biology

摘要 DNA连接酶III。DNA连接酶是一种特殊类型的酶，它通过催化形成磷酸二酯键来促进DNA链连接在一起。它在修复生物体内双链DNA的单链断裂方面发挥着作用。该蛋白已在不同的细胞中用SDS-PAGE与质谱分析相结合进行了研究。 B.端粒酶。端粒酶是一种核糖核蛋白，是一种将DNA序列重复序列添加到DNA链3‘端的酶。用SDS-PAGE和质谱联用对该蛋白的诱导进行了研究。 普列冈。普利冈是一种常用的调味剂(薄荷调味剂)，已知在高剂量下是有毒的。在显微镜下，已经证明当大鼠被给予高剂量的这种化学物质时，会发生肾小球病变。为了解决受影响大鼠肾脏的组成问题，我们正在使用质谱仪来鉴定存在的蛋白质，并确定哪些蛋白质受到这种化学物质的影响。这项研究应该提供大量的信息，有助于理解这种化学物质的机制。

Summary A. DNA Ligase III. DNA Ligase is a specific type of enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond. It plays a role in repairing single-strand breaks in duplex DNA in living organisms. This protein has been investigated in different cells by SDS-PAGE coupled to mass spectrometric analyses. B. Telomerase. Telomerase is a ribonucleoprotein that is an enzyme that adds DNA sequence repeats to the 3' end of DNA strands. Induction of this protein was investigated by SDS-PAGE coupled to mass spectrometric analyses. C. Pulegone. Pulegone is a commonly-used flavoring agent (mint flavoring) and is known to be toxic in high doses. Using microscopy, it has been shown that glomerulopathy can occur when rats are given high doses of this chemical. To address the composition of the kidney of affected rats, we are using mass spectrometry to identify the proteins present as well as determine which proteins are affected by this chemical. This study should provide a significant amount of information that can help in understanding the mechanism of this chemical.