Clinical and Microbiology Laboratory Core

临床和微生物学实验室核心(Micro Core B)支持东南部科学和技术研究所的4个项目 合作研究中心，为其他地方、国家和国际机构提供广泛的支持基础 STI研究项目。我们将对淋病奈瑟菌进行实验性人类感染研究，以 确定淋球菌抗菌外排泵、脂低聚糖结构和 阶段变化(项目3，Shafer)；菌毛相关蛋白(项目4，Thomas)；铁调节的粘附素 (项目2，Comelissen)；和肽聚糖释放(约瑟夫·迪拉德，大学)。威斯康星州)治疗尿路感染 在男人身上。此外，还将从实验感染的受试者身上收集临床样本进行分析，以 评估对实验性淋球菌感染的先天免疫反应(项目5，邓肯)。为了方便 淋病奈瑟菌中铁调节表面蛋白的鉴定与鉴定 作为粘附素，Micro Core将协调维吉尼亚大学项目2中Comelissen博士之间的互动 英联邦大学和北卡罗来纳-杜克大学迈克尔·胡克蛋白质组学中心，位于联合国开发计划署教堂 希尔校园。此外，微核将作为临床和生物学的储存库 来自各种研究的样本，用于未来的性传播感染检测。我们将继续评估新的诊断测试 性传播病原体。特别是，我们将评估阴道毛滴虫基因分型系统。 (在以前的NC STI-TM CRC中作为一个项目开发)使用来自艾滋病毒感染妇女的最近样本 滴虫病参与了海湾南部STI CRC和阴道毛滴虫正在进行的临床研究 在秘鲁UW STI CRC最近完成的一项研究中，从妇女中分离出了这些病毒。其他活动 将包括评估和使用最近接触精液的生物标记物的测试，包括检测 前列腺特异性抗原。此外，Micro Core将继续执行STI诊断测试 支持临床研究研究。

The Clinical and Microbiology Laboratory Core (Micro Core B) supports 4 Projects in the Southeastern STI Cooperative Research Center and provides a broad base of support for other local, national and international STI research projects. We will conduct experimental human infection studies with Neisseria gonorrhoeae to determine the requirements for the gonococcal antimicrobial efflux pump, lipooligosaccharide structures and phase variation (Project 3, Shafer); pilus-associated proteins (Project 4, Thomas); iron-regulated adhesins (Project 2, Comelissen); and peptidoglycan release (Joseph Dillard, Univ. of Wisconsin) for urethral infection in men. In addition, clinical specimens from experimentally infected subjects will be collected for analysis to evaluate innate immune responses to experimental gonococcal infection (Project 5, Duncan). To facilitate the identification and characterization of iron-regulated surface proteins in N. gonorrhoeae that may function as adhesins, the Micro Core will coordinate interactions between Dr. Comelissen in Project 2 at the Virginia Commonwealth University and the UNC-Duke Michael Hooker Proteomics Center, located on the UNCChapel Hill campus. In addition, the Micro Core will serve as a repository of clinical and biological specimens from a variety of studies for future STI testing. We will continue to evaluate new diagnostic tests for sexually transmitted pathogens. In particular, we will evalute a T. vaginalis genotyping system (developed as a project in the previous NC STI-TM CRC) using recent specimens from HIV-infected women with trichomoniasis participating in an ongoing clinical study in the Gulf South STI CRC and T. vaginalis isolates from women in a recently completed study conducted by the UW STI CRC in Peru. Other activities will include the evaluation and use of tests for biomarkers of recent semen exposure, including detection of prostate-specific antigen. In addition, the Micro Core will continue to perform STI diagnostic testing in support of clinical research studies.