MHC and TCR reagents core

核心B将提供各种IVIHC和TCR试剂，并将使用这些试剂进行实验，以支持研究项目。将提供的常规服务包括生产纯化的重组MHC和TCR蛋白，用于四聚体染色和亲和力测量，四聚体试剂的测试和质量控制，MHC-肽和MHC-TCR结合亲和力和动力学的生物物理测量，以及MHC-肽复合物的结构表征。这个核心部分也将是发展性的。将提供的开发服务包括生产用于表征T细胞交叉反应性的MHC异源二聚体，以及基于目前对MHC II肽相互作用的理解创建用于分析II类限制性CD 4 T细胞的改进试剂。这项工作的具体目的是产生双功能MHC二聚体以检测交叉反应性T细胞，开发用于I-A（B）的II类MHC四聚体技术，测量MHC-TCR对的结合亲和力和动力学，并提供NIH四聚体设施无法获得的I类MHC四聚体试剂。核心B提供的服务将由项目1（Swain）、项目2（Welsh）、项目3（Tsuda）和项目4（Selin）以及核心C（Huseby）使用，

Core B will provide a variety of IVIHC and TCR reagents, and will perform experiments using these reagents, in support of the research projects. Routine services that will be provided include production of purified recombinant MHC and TCR proteins for tetramer staining and affinity measurements, testing and quality control of tetramer reagents, biophysical measurements of MHC-peptide and MHC-TCR binding affinity and kinetics, and structural characterization of MHC-peptide complexes. This core will also be partly developmental. Developmental services that will be provided include production of MHC heterodimers for characterization of T cell cross reactivity, and creation of improved reagents for analyzing class ll-restricted CD4 T cells based on current understanding of MHC II peptide interactions. The specific aims of this work are to generate bifunctional MHC dimers to detect cross-reactive T cells, to develop class II MHC tetrarner technology for l-A(b), to measure binding affinity and kinetics for MHC-TCR pairs, and to provide class I MHC tetramer reagents not available from the NIH tetramer facility. Services provided by core B will be used by Project 1 (Swain), Project 2 (Welsh), Project 3 (Tsuda), and Project 4 (Selin), and also by Core C (Huseby),