Hedgehog signaling and signal transduction

项目摘要/摘要 Hedgehog信号与许多癌症有关，并已被证明是一个有用的靶点 抗癌治疗。以前的工作，包括我的实验室的主要贡献，已经建立了 刺猬蛋白是动物发育的主要调节因子之一，在大多数组织中发挥着关键作用。 以及无脊椎动物和脊椎动物的器官。使用新开发的试剂和方法打开 研究HH信号的新方法，本申请中提出的工作将识别和表征HH信号 产生HH的细胞用来制备和传递HH蛋白的过程，HH接收的过程 细胞用来准备和处理它的到来，以及调节Ci形式和活动的过程， 负责HH途径输出的转录因子。这些问题对于理解 如何开发控制HH活性的新的和更好的治疗方案。

Project Summary/Abstract Hedgehog signaling has been implicated in many cancers and has been shown to be a useful target for anti-cancer therapy. Previous work, including key contributions from my lab, have established that the Hedgehog protein is one of the major regulators of animal development, playing key roles in most tissues and organs in both invertebrates and vertebrates. Using newly developed reagents and methods that open new ways to study Hh signaling, the work proposed in this application will identify and characterize the processes that Hh-producing cells use to prepare and deliver Hh protein, the processes that Hh-receiving cells use to prepare for and handle its arrival, and the processes that regulate the form and activity of Ci, the transcription factor that is responsible for Hh pathway output. These issues are critical to understanding how new and better therapeutic regimens controlling Hh activity can be developed.