Pannoramic 250 BF Flash Tissue Microarray system

描述（由申请人提供）：转化病理学共享资源（TPSR）正在申请高通量自动化组织微阵列系统，TMA grandmaster -全景250系统（TMAG/P250）的资金。该系统的设计具有出色的生产力，并具有最高的阵列制备精度。除了劳动密集型组织微阵列（TMA）制备的自动化和标准化的提高之外，TMAG/P250集成还有其他主要好处。P250产生供体块的H&E染色切片的数字图像，该图像被导入TMAG并与供体块的图像共同注册。这有效地将组织核心选择和供体块注释整合到一个步骤中，这相当于节省了大量时间，更重要的是提高了核心选择的准确性。使用Case Center软件，可以从任何互联网接入点查看和注释组织核心收集的数字幻灯片图像，这使得协作机会几乎是无限的。后处理后，TMAG的元数据可以导出为连续的数据流，从而消除了数据输入错误并便于快速图像分析。没有其他类似的高通量系统可供范德比尔特研究人员使用。tma是一种强大的工具，用于分析大型患者群体的靶蛋白，同时最大限度地减少有价值组织的消耗。一张载玻片上的数百个组织样本可以在统一的实验条件下进行测试，防止测定间的差异。蛋白质组学、基因组学和质谱学领域的巨大成功取决于组织基础分析的验证，并导致对tma的需求增加。使用手动比彻阵列在TPSR中构建了黑色素瘤、结直肠癌、胰腺癌、肝细胞癌、甲状腺癌、乳腺癌、前列腺癌、头颈癌和肺癌的tma。手工构建和分析TMA是繁琐和耗时的，并且限制了它们的使用。该应用程序的主要用户是非常成功的nih资助的科学家，他们以前有TMA的经验，目前正在使用TMA，或者已经计划了需要TMA准备的项目。这些用户代表了从事临床和基础研究的范德比尔特研究者的横截面，他们的兴趣广泛，包括黑色素瘤、肺癌、肾脏疾病、传染病、前列腺癌和胃肠病学。TMAG/P250系统将通过在大样本组上提供高通量筛选新型生物标志物的平台来加速它们的发现速度。

DESCRIPTION (provided by applicant): The Translational Pathology Shared Resource (TPSR) is requesting funds for a high throughput automated tissue microarray system, the TMA Grandmaster-Panoramic 250 system (TMAG/P250). This system is designed for outstanding productivity partnered with the highest possible precision in array preparation. In addition to automation of labor intensive tissue microarray (TMA) preparation and improved standardization, there are other major benefits to the TMAG/P250 integration. The P250 produces a digital image of an H&E stained section of the donor block that is imported into the TMAG and co-registered with an image of the donor block. This effectively consolidates tissue core selection and donor block annotation into one step which equates to significant time savings and more importantly increased accuracy in core selection. Using Case Center software, the digital slide images can be viewed and annotated for tissue core collection from any internet access point which makes collaboration opportunities virtually infinite. Post-processing, the metadata from the TMAG can be exported for a continuous dataflow, which eliminates date entry errors and facilitates rapid image analysis. There is no other comparable high throughput system available for Vanderbilt investigators. TMAs represent a powerful tool for analyzing large patient cohorts for target proteins while minimizing the consumption of valuable tissue. Hundreds of tissue samples on one slide can be tested under uniform experimental conditions preventing interassay variability. Considerable success in the fields of proteomics, genomics, and mass spectrometry depends on validation in tissue based assays and has resulted in increased demand for TMAs. TMAs for melanoma, colorectal, pancreatic, hepatocellular, thyroid, breast, prostate, head and neck, and lung cancers have been constructed in the TPSR using a manual Beecher arrayer. Manual TMA construction and analysis is tedious and time consuming and has limited their use. The major users in this application are highly successful NIH-funded scientists that have previous experience with TMAs, currently utilize TMAs, or have planned projects that require TMA preparation. These users represent a cross section of Vanderbilt investigators engaged in clinical and basic research with diverse interests including melanoma, lung cancer, renal disease, infectious disease, prostate cancer, and gastroenterology. The TMAG/P250 system will accelerate their rate of discovery by providing a platform for high through-put screening of novel biomarkers on large sample groups. .