A Database of Mammalian Neuronal Cell Types

摘要 神经细胞类型是大脑功能的基石，也是细胞之间的差异 类型对于了解神经系统疾病至关重要。新的遗传和基因组 越来越多的资源可用于识别和操作中的特定单元类型 那只老鼠。我们将创建一个通往这些试剂的统一门户和一个平台 与他们整合和共享所获得的数据，以加强在困难问题上的进展 识别哺乳动物大脑的神经细胞类型的问题。我们将实施 一个可搜索的、免费共享的开放源码资源和实验数据库 结果。我们将注释~90个目标细胞类型的解剖位置 现有司机/记者通过将个别部分与艾伦大脑进行比对而产生的压力 参考Atlas，并将使用新的慢病毒增强子陷阱开发新菌株 在老鼠身上的策略。对于细胞类型的子集，我们将执行基因组-基因表达 分析以增强对神经元之间关系的这种无偏见的度量的洞察力 单元类型。我们将把细胞类型数据库和改进的地图集查看器与工具集成在一起 用于查看特定细胞类型的形态、生理和基因表达数据，并将 将这些数据与其他可用的脑图谱和基因组数据库联系起来。有能力 自由搜索和比较细胞类型特定表型和基因表达数据将 使科学界的成员能够发展出关于 特定细胞表型的分子基础和改进分类方案 神经细胞类型。

Abstract Neuronal cell types are the building blocks of brain function and differences between cell types are critical to understanding nervous system disease. New genetic and genomic resources are increasingly available for identifying and manipulating specific cell types in the mouse. We will create a unified gateway to these reagents and a platform for integrating and sharing data obtained with them to enhance progress on the difficult problem of identifying the neuronal cell types of the mammalian brain. We will implement a searchable, freely shared open source database of resources and experimental results. We will annotate the anatomical location of targeted cell types within each of ~90 existing driver/reporter strains by aligning individual sections to the Allen Brain Reference Atlas and will develop new strains using a novel lentiviral enhancer trap strategy in mice. For a subset of cell types we will perform genome-gene expression profiling to enhance insights from this unbiased metric of relationships between neuronal cell types. We will integrate the cell type database and improved atlas viewer with tools for viewing cell type-specific morphology, physiology and gene expression data, and will link these data with other available brain atlas and genomic databases. The ability to freely search and compare cell-type specific phenotypes and gene expression data will enable members of the scientific community to develop specific hypotheses about the molecular bases of specific cellular phenotypes and to improve schemes for classifying neuronal cell types.