Scientific Core: Bacillus anthracis Anthrax Toxin Core

炭疽毒素的作用和将毒素成分用作炭疽疫苗的可能性，是本提案中许多拟议项目的核心。这个核心将为研究人员提供高纯度的炭疽毒素蛋白，可以在大小规模的研究中始终如一地使用。毒素核心会提纯大量的PA、LF和EF，这是不含内毒素的。核心将测试一批毒素的相对活性，并将提供归一化的毒素批次，因此研究人员可以在该项目的五年内进行多项研究，对该试剂的一致性充满信心。该核心还将存储和编目PA、LF和EF，并雇用一名技术人员来监督毒素的纯化、表征和分发。

The role of anthrax toxin and the potential for using components of the toxin as an anthrax vaccine, are central to many of the proposed projects in this proposal. This core will provide investigators with highly purified anthrax toxin proteins that can be used consistently in large and small-scale studies. The toxin core will purify large amounts of PA, LF, and EF, that is LPS-free. Batches of toxin will be tested by the core for relative activity and normalized batches of toxin will be provided, so investigators can pursue multiple studies over the five years of this project confident in the consistency of this reagent. This core will also store and catalog PA, LF, and EF and employee a technician that will oversee the purification, characterization, and distribution of the toxin.