Cellular and Molecular Biology

核心C：细胞与分子生物学-金莹，博士-派 这个核心在神经生物学和解剖学系内已经存在了10年，以促进 脊髓损伤部位和移植体内细胞和组织的分子分析。最重要的是，核心 为单个项目中的移植实验准备细胞。所有项目都将使用细胞和分子 核心。该设施分离、扩增和储存原代成纤维细胞、神经干细胞和细胞系 必填项。我们通过病毒转导或转基因对细胞进行基因改造，并为所有 核心中使用的细胞和试剂。细胞和分子核心的工作人员为 实验设计和数据分析。核心开发了一个计算机化的订购系统，其中 个别研究人员和工作人员要求对照、标记或转基因细胞进行移植。我们的 记录显示，在过去的5年里，核心提供了超过3x10^的细胞，包括未经修改的和 修饰成纤维细胞，神经元和神经胶质限制性前体(NRP和GRP)，神经上皮细胞(NEP) 以及来自永生化细胞系的细胞。核心长期维持病毒和转导的细胞库存 存储，并作为数据库和cdna克隆、载体、病毒和细胞的储存库。高效和 经济高效地订购组织培养和分子生物学用品以及大规模测试新的 血清和分子分析所需的改进的生长介质、酶、试剂盒或设备(热循环仪， Q-PCR)是关键的核心功能。我们还在不断开发新的改进的质粒和病毒 最近又将腺病毒载体(AAV)作为选项添加到我们的计划中。这将是 允许我们将具有促生长和治疗因子的移植物神经干细胞特异性地转染成 在受伤地点或附近交付的。

CORE C: Cell and Molecular Biology - Ying Jin, Ph.D. - PI This Core has existed for 10 years within the Department of Neurobiology and Anatomy to facilitate the molecular analysis of cells and tissues in the SCI site and within transplants. Most importantly, the core prepares cells for grafting experiments in the individual projects. All projects will use the Cell and Molecular Core. The facility isolates, expands and stores primary fibroblasts, neural stem cells and cell lines as required. We genetically modify cells by viral transduction or transfection and provide quality control for all cells and reagents used in the core. The staff of the Cell and Molecular Core provides assistance for experimental design and data analysis. The core has developed a computerized ordering system in which individual investigators & staff request control, labeled or genetically modified cells for transplantation. Our records show that over the past 5 years the core has provided over 3 x 1 0 ^ cells, including unmodified and modified fibroblasts, neuronal and glial restricted precursors (NRP and GRP), neuroepithelial cells (NEP) and cells from immortalized cell lines. The core maintains viruses and transduced cell stocks in long-term storage and serves as a database and repository for cDNA clones, vectors, viruses and cells. Efficient and cost-effective ordering of tissue culture and molecular biology supplies as well as large scale testing of new sera and improved growth media, enzymes, kits or equipment needed for molecular analysis (thermocyclers, Q-PCR) are key core functions. We are also continuously developing new improved plasmids and viruses and have recently added and adeno-associated virus vector (AAV) as an option to our program. This will allow us to specifically transfect graft neural stem cells with growth promoting and therapeutic factors to be delivered at or near the site of injury.