Nonhuman Primate IVIS Spectrum Imaging System

描述（由申请人提供）：加州国家灵长类动物研究中心转化体内成像项目，是计算成像核心的一个组成部分，为美国国立卫生研究院资助的研究人员提供最先进的体内成像服务和专业知识。这个已建立的项目为研究人员提供了独特的机会，可以将非侵入性成像纳入他们的研究项目，并研究人类健康和疾病的猴子模型。本提案的目标是升级和替换一种光学成像系统，该系统将不再由制造商/供应商提供支持或服务，从而使许多正在进行和计划中的NIH研究处于危险之中。替换的IVIS光谱成像系统将维持在这一已建立的核心服务结构内，该结构在满足美国国立卫生研究院支持的全国研究人员的体内成像需求方面非常有效。该成像系统将位于一个专用的成像套件中，在一个安全的环境中，并具有对非人灵长类动物进行研究所需的职业健康和安全条件。IVIS光谱成像系统可用于反射和传输模式，包括一个高灵敏度的背薄背照CCD相机，一个带荧光测量滤波器的宽带激发源，用于多光谱检测的可选滤波器，以及控制数据采集的软件，用于光谱分离，3D信号定位和量化，以及图像显示和分析。除了正在进行的猴子纵向研究，重点是基因转移/基因治疗、干细胞移植、再生医学、组织工程、传染病和寿命健康，该系统将作为进一步开发和应用这些新型成像技术的平台，并跨越研究领域。这个替代系统将确保加州国家灵长类动物研究中心正在进行的和新的研究机会能够继续满足国家卫生研究院资助的研究人员在当地、地区和国家的需求，这些研究人员专注于更好地了解人类健康和疾病的方法。

DESCRIPTION (provided by applicant): The California National Primate Research Center translational in vivo imaging program, which is a component of the Computational Imaging Core, provides state-of-the-art in vivo imaging services and expertise to NIH- funded investigators nationally. This established program includes unique opportunities for investigators to incorporate noninvasive imaging in their research programs, and to study monkey models of human health and disease. The goal of this proposal is to upgrade and replace an optical imaging system that will no longer be supported or serviced by the manufacturer/supplier placing many NIH studies in progress and planned at risk. The replacement IVIS Spectrum Imaging System will be maintained within this established core service structure, which has been highly effective in meeting the in vivo imaging needs of NIH-supported investigators nationwide. This imaging system will be located within a dedicated imaging suite in a secure environment, and with the required conditions for occupational health and safety for research with nonhuman primates. The IVIS Spectrum Imaging System can be used in both reflectance and transmission modes and includes a highly sensitive back-thinned, back-illuminated CCD camera, a broad band excitation source with filters for fluorescence measurements, selectable filters for multispectral detection, and software to control data acquisition, for spectral unmixin, 3D signal localization and quantification, and image display and analysis. In addition to ongoing longitudinal studies in monkeys focused on gene transfer/gene therapy, stem cell transplantation, regenerative medicine, tissue engineering, infectious diseases, and lifespan health, the system will serve as the platform for further development and application of these novel imaging techniques in monkeys, and span research domains. This replacement system will ensure ongoing and new research opportunities at the California National Primate Research Center can continue to meet the needs of NIH- funded investigators locally, regionally, and nationally that are focused on ways to better understand human health and disease.