Genomics Core

本申请中研究提案的目标是在全球范围内确定 支持αβ与γδ血统选择的机制。其中提出的许多实验 应用涉及全基因组分析和对从这种分析中获得的数据的解释。 基因组学核心的目的是向实验室提供以下服务，以允许 建议的研究有待完成。基因组学核心将执行HIC、ChlP-Seq和RNA-Seq 分析，然后是生物信息学分析。因此，全基因组分析的整个光谱 担心这一POL将在UCSD基因组学核心设施进行。 为什么要有集中化的基因组学核心？有三个原因：(1)除了默尔实验室，没有一个 参与的实验室拥有ChlP-Seq和RNA-Seq方面的专业知识。(2)壮族、威斯族和 Zuniga-Pflucker实验室无法获得生物信息基础设施和分析工具 已经在加州大学圣迭戈分校成立了。(3)基因组学核心将允许我们标准化ChlP-Seq 和RNA-Seq分析。这意味着使用相同的抗体、相同的批号和相同的方法 从而允许对将导出的不同结合模式进行仔细且一致的比较 来自不同的研究。(4)基因组学核心将为访问研究员提供培训。 因此，我们设想其他实验室的研究生和博士后研究员将访问 加州大学可持续发展分校，以便熟悉全球方法和分析。因此，一个不可分割的部分 我们的方法是向来自不同机构的人员提供这些全球分析工具和 提供培训。

The objectives of the research proposals in this application are to determine at a global scale the mechanisms that underpin the αβ versus γδ lineage choice. Many of the experiments proposed in the application involve genome-wide analyses and interpretation of the data obtained from such analyses. The purpose of the Genomics Core is to provide the laboratories with the following services that will permit the proposed studies to be completed. The Genomics Core would perform HiC, ChlP-Seq and RNA-Seq analyses, followed by bioinformatics analysis. Thus the entire spectrum of genome-wide analyses that concerns this POl would be performed at the UCSD Genomics Core Facility. Why a centralized Genomics Core? There are three reasons: (1) Except for the Murre laboratory, none of the participating laboratories have expertise in ChlP-Seq and RNA-Seq. (2) The Zhuang, Wiest and Zuniga-Pflucker laboratories do not have access to the bioinformatic infrastructure and analytical tools that have been established at UCSD. (3) The genomics core would permit us to standardize the ChlP-Seq and RNA-Seq analysis. This means using the same antibodies, same lot number and the same approach thereby allowing a careful and consistent comparison of the different binding patterns that will be derived from the various studies. (4) The Genomics Core will provide training to visiting research fellows. Thus, we envision that graduate students and postdoctoral fellows from the other laboratories would visit UCSD in order to become familiar with global approaches and analyses. Accordingly, an integral part of our approach is to provide personnel from different institutions access to these global analysis tools and provide training.