NIAMS Light Imaging Facility

在过去一年中，来自14个科、实验室或分支机构的研究人员使用了该设施，26名研究人员接受了使用一种或多种仪器的新培训。 地雷影响调查工作人员协助设施用户应用要求更高的显微技术（例如荧光寿命成像），以提高图像质量，并准备这些图像供出版。参考书目中列出了从此类帮助中受益的NIAMS出版物和/或显示我们仪器上收集的图像。地雷影响调查工作人员还帮助评价设施用户感兴趣的新仪器。 从今年到去年，在LIS进行的显微镜检查有助于扩大NIAMS几个研究目标领域的知识，包括： i）了解免疫系统及其疾病（Kouzine等人，2013; Layh-Schmitt等人，等人，2013）; ii）了解软骨及其疾病（Gabay等，2013）; iii）了解皮肤和毛发发育和疾病（Okano等人，2013年度） iv）理解和治疗肌肉疾病（Spampanato等人，2013）和肌肉组织（Oddoux等人，出版中）。 通过收购Leica Slidescanner 400 SCN，仪器库得到了升级。该仪器可扫描带有组织学样本或荧光标记组织切片的整个载玻片。对于组织学样本，载玻片扫描仪以自动模式工作。它产生高质量的图像，可以通过大范围的放大在线可视化，“一个谷歌地图。“荧光标记样品的扫描在设置和扫描时间方面要求更高，并且不是自动化的。该仪器由LIS工作人员专门处理。在光成像科以外的6个实验室/分支机构中，共为13名用户扫描了747张载玻片。 我们还收购了Incucyte Zoom，这是Incucyte的新版本（一种适合组织培养箱的显微镜，用于在数天或数周内对细胞进行延时成像）。变焦允许对标记有2种荧光标记物（红色和绿色）的样本进行成像。到目前为止，光成像科以外的4个实验室/分支机构的7名用户使用了该系统。 Slidescanner和Incucyte都属于新一代自动化仪器，可以从其他建筑物的实验室或办公室甚至家中远程访问图像和实时分析。 我们还升级了SP 5共聚焦装置（Leica），配备了更灵敏的内部检测器。 设施工作人员已经给了一组高中学生参观设施。

Researchers from 14 Sections, Laboratories, or Branches have used the Facility during the past year and 26 researchers have been newly trained to use one or more of the instruments. LIS staff have assisted Facility users to apply more demanding microscopy techniques (Fluorescence Lifetime Imaging, for example), to improve the quality of their images, and to prepare these images for publication. NIAMS publications which have benefited from such help and/or show images collected on our instruments are listed in the bibliography. LIS staff have also helped with evaluation of new instruments that Facility users are interested in. Microscopy done in LIS this to to past year has contributed to the expansion of knowledge in several NIAMS research target areas to include: i) understanding the immune system and its diseases (Kouzine et al., 2013; Layh-Schmitt et al., 2013; Aubert, P. et al. , 2013); ii) understanding cartilage and its diseases (Gabay et al, 2013); iii) understanding skin and hair development and diseases (Okano et al., 2013) iv) understanding and treating muscle diseases (Spampanato et al., 2013) and muscle organization (Oddoux et al., in press). The pool of instruments has been upgraded by the acquisition of the Leica Slidescanner 400SCN. This instrument performs scanning of whole slides with histology samples or fluorescently labeled tissue slices. For histology samples the Slidescanner works in automated mode. It produces high quality images that can be visualized online through a large range of magnifications, "a la Google map." Scanning of fluorescently labeled samples is much more demanding in setup and scanning time, and is not automated. The instrument is handled by LIS staff exclusively. In all 747 slides have been scanned for 13 users in 6 Laboratories/Branches outside of the Light Imaging Section. We have also acquired the Incucyte Zoom, the new version of the Incucyte (a microscope that fits inside a tissue culture incubator for time-lapse imaging of cells over periods of days or weeks). The Zoom allows imaging of samples labeled with 2 fluorescent markers (red and green). It has been used so far by 7 users in 4 Laboratories/Branches outside of the Light Imaging Section. Both Slidescanner and Incucyte belong to a new generation of automated instruments that produce images and on-the-fly analysis accessible remotely, from labs or offices in other buildings or even from home. We have also upgraded the SP5 confocal setup (Leica) with more sensitive internal detectors. Facility Staff have given a tour of the Facility to a group of high-school students.