Midcareer Investigator Award in Patient-Oriented Research (K24)

目标。近期和长期目标是：1)成为翻译、临床和医疗领域的领导者 与肝病相关的基础研究；以及2)培养工作于 胃肠道和肝脏疾病。1月1日致力于加强医科学生的教育培训， 住院医生、研究员和初级教员参与研究，促进以患者为导向的研究的发展。 研究项目。在这份申请中，我包括了两个以患者为中心的研究项目，这两个项目源于我的 目前的丙型肝炎病毒中心赠款和使用Virahep-C队列的ROL赠款。这两个截然不同的，很有特点的 纳入队列是为了展示未来受训人员可以进行的各种研究。这个 我们研究的中心目标是了解控制丙型肝炎病毒感染康复的免疫因素 自发的或治疗诱导的。这里提出的新研究的目的是回答其他 在实施资助项目过程中出现的问题。 发展规划。到目前为止，我指导过的大多数人(12人中的11人)都在追求学术 事业。来自此K24的支持将把我从临床和管理责任中解放出来，并允许我 指导更多的实习生，并探索杰出的科学领域内的许多合作机会 加州大学洛杉矶分校的社区。 研究环境。这项提案中概述的研究将在罗森博士的实验室进行，面积为2707平方英尺 UCDS研究综合体10楼2.实验室内有生物危害层流罩、细胞培养 二氧化碳培养箱、倒置和竖立显微镜、安全壳离心机、-70℃和液氮 冰柜和标准的聚合酶链式反应设备。该实验室还配备了一台FACS Aria、Luminex系统和一台自动AID Elispot阅读器。邻近的空间可用于分子生物学和蛋白质表达工作。种类繁多 可通过GL分部和科罗拉多州艾滋病研究中心(CFAR)获得共享设备， 包括流式细胞仪和细胞分选仪(流式细胞仪核心)、高速和超速离心机、伽马和 贝塔计数器、细胞收割机、荧光显微镜、FPLC和自动ABI 377测序仪。

Goals. Immediate and long-term objectives are: 1) to be established as a leader in translational, clinical, and basic research related to liver disease; and 2) to train the next generation of physician-scientists working in gastrointestinal and liver disease. 1 am committed to enhancing the educational training of medical students, residents, fellows, and junior faculty members in research and facilitate the growth of patient-oriented research. Research Project. In this application, 1 have included two patient-oriented research projects that stem from my current HCV Center Grant and an ROl grant using the Virahep-C cohort. These two distinct, well-characterized cohorts are included to demonstrate the variety of research that would be available for future trainees. The central goal of our research is to understand the immune factors that govern recovery from HCV infection, either spontaneously or therapy-induced. The purpose ofthe new research proposed here is to answer additional questions that have arisen in the course of carrying out the funded projects. Development plan. To date, the majority of individuals I have mentored (11 of 12) have pursued academic careers. Support from this K24 will release me from clinical and administrative responsibilities and allow me to mentor of more trainees and explore the many collaborative opportunities within the outstanding scientific community at UCHSC. Research environment. The studies outlined in this proposal will be performed in Dr. Rosen's lab, 2,707 sq ft on the 10th floor of UCDs Research Complex 2. The laboratory contains biohazard laminar flow hoods, cell culture C02 incubators, inverted and upright upright microscopes, containment centrifuges, -70 C and liquid nitrogen freezers, and standard PCR equipment. The lab also houses a FACS Aria, Luminex system, and an AID automated ELISPOT reader. Adjacent space is available for molecular biology and protein expression work. A wide variety of shared equipment is available through the Gl Division and the Colorado Center for AIDs Research (CFAR), including flow cytometers and cell sorters (Flow Cytometry Core), high speed and ultracentrifuges, gamma and beta counters, cell harvesters, fluorescent microscope, FPLC, and automated ABI 377 sequencer.