Acquisition of a High Resolution Mass Spectrometer for the University of Cincinna

描述（由申请人提供）：该提案要求提供资金，以获得高分辨率傅里叶变换质谱仪和相关的液相色谱系统，以支持辛辛那提大学研究人员的NIH资助研究。这种新仪器旨在支持NIH资助的正在进行的健康相关问题研究，如抗生素耐药性、动脉粥样硬化、癌症、糖尿病、传染病和肥胖症。具体而言，通过此次收购将实现以下类型的分析：a）代谢通量测量，以识别心脏代谢疾病和各种癌症中的关键代谢途径; B）代谢物识别，以支持在代谢通量测量期间揭示的代谢途径的识别;以及c）溶血磷脂和胆固醇代谢途径的代谢组学表征。辛辛那提大学NIH支持的一组关键研究人员将直接受益于这些新功能。此外，这些新功能将刺激这些研究人员和校园内的其他生物医学研究人员检查将从该仪器中受益的其他实验。参与此请求的NIH资助的研究人员需要专用的LC-MS/MS或高分辨率ESI/APCI-MS/MS功能，以实现与其资助项目相关的目标。PI在质谱领域活跃了20多年，将负责所需仪器的管理监督。他将向由大学内部科学家组成的外部咨询委员会报告。咨询委员会将确定日程安排的优先次序，与PI和会计人员合作确定成本回收率，并将作为预测所需设备未来使用情况的资源。设备将存放在辛辛那提大学质谱设施中。这3,500平方米。英尺该设施配备有生物分子表征所需的辅助仪器。仪器将由两名博士操作和维护-一级的设施科学家，其中一名科学家被指定为主要操作员。

DESCRIPTION (provided by applicant): This proposal requests funds to acquire a high resolution Fourier transform mass spectrometer and associated liquid chromatography system to support the NIH-funded research of investigators at the University of Cincinnati. This new instrumentation is intended to support on- going NIH-funded research into health-related issues such as antibiotic resistance, atherosclerosis, cancer, diabetes, infectious diseases and obesity. Specifically, the following types of analyses will be enabled by this acquisition: a) Metabolic flu measurements to identify key metabolic pathways in cardiometabolic diseases and various cancers; b) Metabolite identification to support identification of metabolic pathways revealed during metabolic flux measurements; and c) metabolomic characterization of lysophospholipid and cholesterol metabolic pathways. A key group of NIH-supported investigators at the University of Cincinnati will directly benefit from these new capabilities. Moreover, these new capabilities will stimulate these researchers and other biomedical investigators on campus to examine additional experiments that will benefit from this instrument. The NIH-funded researchers participating in this request require dedicated LC-MS/MS or high-resolution ESI/APCI-MS/MS capabilities to accomplish goals associated with their funded projects. The PI, who has been active in the field of mass spectrometry for over 20 years, will be responsible for the managerial oversight of the requested instrumentation. He will report to an external advisory board composed of scientists from within the university. The advisory board will establish scheduling priorities, work with the PI and accounting personnel to establish cost-recovery rates, and will serve as a resource for projecting future use of the requested equipment. The equipment will be housed in the University of Cincinnati Mass Spectrometry Facility. This 3,500 sq. ft. facility is equipped with the ancillary instrumentation required for biomolecule characterization. The instrumentation will be operated and maintained by two Ph.D.-level facility staff scientists, with one of these scientists being designated as the primary operator.