Development of laser spectroscopic methods for quantification of 14C

描述（由申请人提供）：我们建议开发一种桌面大小的光谱方法，使用称为腔衰荡光谱的技术，以定量毫克大小的生物样品中的14 C，其水平低至天然同位素丰度。这一系统的购置和操作成本将低得多，最终可能取代目前最先进的技术-加速器质谱法。这种方法将导致一个新的模式，14 C分析，允许进行研究的代谢，处置，命运和质量平衡的14 C标记的治疗实体的药物开发，潜在的有毒物质的风险评估，并分析生物标志物的个性化医疗。考虑到预期的成本和操作的简单性，这种新技术将使更多的访问高灵敏度14 C分析比现在可用的任何生物医学或生物化学实验室可以负担得起操作这样的仪器。

DESCRIPTION (provided by applicant): We propose to develop a table-top sized spectroscopic method to quantify 14C in milligram sized biological samples at levels down to the natural isotopic abundance, using a technique called Cavity Ring Down Spectroscopy. This system will be much cheaper to acquire and to operate, and could eventually replace the current state of the art technology -- accelerator mass spectrometry. This approach will lead to a new paradigm for 14C analysis in allowing studies to be carried out on the metabolism, disposition, fate and mass balance of 14C-labeled therapeutic entities for drug development, potential toxicants for risk assessment, and analysis of biomarkers for personalized medicine. Given the expected cost and simplicity of operation, this new technique will enable much greater access to high sensitivity 14C analysis than now currently available as any biomedical or biochemistry laboratory could afford to operate such an instrument.