Hybrid Nanopore Platform for DNA Sequencing

描述（由申请人提供）：拟议研究的目的是展示混合纳米孔结合自上而下的图案和纳米制造与自下而上的生物组装。现有的纳米孔测序，特别是脂质双层中的生物纳米孔，以降低并行化和数据吞吐量为代价，实现了高速度和高读取长度。该平台的脆弱性也使其在商业化方面不太实用。该项目的主要目标是建立一种生物-固体混合结构，该结构可以以强大的自动化方式高效自组装。我们的初步研究已经确定了可行的生物通道载体，可以组装在~10nm的固态纳米孔上。建议的工作将集中在纳米孔蛋白掺入的演示，自限制通道插入，以及用混合平台演示生物纳米孔活性。该平台保留了生物纳米孔系统的速度和读取长度特征，同时通过利用现有的半导体技术显著提高了并行性和系统稳定性。

DESCRIPTION (provided by applicant): The objective of the proposed research is to demonstrate hybrid nanopores combining top-down patterning and nanofabrication with bottom-up biological assembly. Existing nanopore sequencing, especially biological nanopores in lipid bilayers, achieve high speed and read length at the cost of decreased parallelization and data throughput. The fragility of the platform also makes it less practical in commercialization. The primary goal of this project is to establish a hybrid biological-solid state structure that can be self-assembled in a robust automated manner with high efficiency. Our preliminary research has identified plausible carriers of biological channels that can be assembled onto ~10nm solid-state nanopore. The proposed work will focus on demonstration of nanopore protein incorporation, self-limited channel insertion, and demonstration of biological nanopore activity with the hybrid platform. The proposed platform retains the speed and read length characteristics of biological nanopore systems while dramatically increasing the parallelization and system stability by leveraging established semiconductor technologies.