The Dictyostelium Stock Center

 描述（由申请人提供）：真核生物盘基网柄藻是一种广泛用于研究细胞过程（如细胞运动性、趋化性、信号转导、细胞对药物的反应以及宿主-病原体相互作用）的模式生物。网骨藻的基因组包括与脊椎动物、酵母和微生物基因具有显著同源性的基因，吸引了对各种生物学主题感兴趣的研究人员，如人类疾病、比较基因组学和自我/非自我识别。Dictyosteoblasts研究界从Dicty Stock Center（DSC）中受益匪浅，Dicty Stock Center是一个菌株和质粒的中央储存库，提供了一种具有成本效益的方法，确保菌株广泛可用并保持在标准化条件下。DSC使新的Dictyosteroids研究人员能够获得经过测试的研究材料，并以最低的成本快速启动新的研究项目。知识渊博的DSC工作人员为菌株的有效处理提供技术支持和方案。DSC还极大地受益于其与DictyBase的协同整合，DictyBase是Dictyosteophytes的模式生物数据库（MOD），它提供了菌株和质粒注释的网络界面，并允许在线提交菌株订单。DSC收集转基因的网骨藻和其他种类的社会性变形虫，这些变形虫含有新的表型，很难重新设计。它还提供来自经验丰富的研究人员的表达和基因靶向质粒，这些质粒的重建将是耗时的。此应用程序寻求支持以继续运行此社区资源。本提案的目标有两个：（1）通过继续收集、注释和分发来自网囊藻和相关生物的菌株，重点关注产生表型的突变体和标记菌株，维持、扩大和分发DSC收藏（如GFP融合体）以及有用的质粒、cDNA文库和抗体;（2）创建MyDSC以通过创建个性化用户帐户来提高用户的可用性和实用性，允许用户更改、更新或删除他们的订单，请求不可用的菌株，并留下与每个菌株的生长习性或表型有关的评论;该系统还将促进用户与DSC工作人员之间的通信，从而简化双方的工作流程。实施这些改进将提高DSC工作人员的效率，并大大改善用户的在线体验。

 DESCRIPTION (provided by applicant): The eukaryote Dictyostelium discoideum is a model organism widely used for the study of cellular processes such as cell motility, chemotaxis, signal transduction, cellular response to drugs, and host-pathogen interactions. Dictyostelium's genome includes genes with significant homology to vertebrate, yeast and microbial genes, attracting researchers interested in a wide variety of biological topics such as human disease, comparative genomics, and self/non-self-recognition. The Dictyostelium research community has significantly benefited from the Dicty Stock Center (DSC), a central repository of strains and plasmids that provides a cost-effective means of ensuring that strains are widely available and maintained in standardized conditions. The DSC enables new Dictyostelium researchers to obtain tested research materials and quickly start new research projects at minimal costs. The knowledgeable DSC staff provides technical support and protocols for effective handling of the strains. The DSC also benefits greatly from its synergistic integration with dictyBase, the model organism database (MOD) for Dictyostelium, which provides the web interface for strain and plasmid annotations, and allows strain orders to be submitted online. The DSC collects genetically modified strains of Dictyostelium and other species of social amoebae that contain novel phenotypes and which would be difficult to re-engineer. It also offers both expression and gene-targeting plasmids from experienced researchers that would be time consuming to reconstruct. This application seeks support to continue operating this community resource. The goals of this proposal are twofold: (1) Maintain, expand, and distribute the DSC collection by continuing to collect, annotate and distribute strains from Dictyostelium and related organisms, focusing on mutants that produce phenotypes and marked strains (such as GFP fusions) as well as useful plasmids, cDNA libraries, and antibodies following continuously updated standard operating procedures; (2) Create MyDSC to improve usability and utility for users by creating personalized user accounts, allowing users to change, update or delete their orders, request unavailable strains, and to leave comments pertaining to growth habits or phenotypes for each strain; this system will also facilitate communications between users and DSC staff, easing the workflow on both sides. Implementing these improvements will increase efficiency for DSC staff and greatly improve the online experience for users.