COBRE: Smooth Muscle Plasticity

描述（由申请人提供）：在第三阶段COBRE中，我们将继续发展主题重点：平滑肌可塑性，继续并加强在COBRE第一和第二阶段开发的核心实验室服务。平滑肌是含有多种细胞类型的复杂组织。将这些肌肉作为同质组织处理是评估基因表达和在诸如发育和衰老、糖尿病、重塑、对炎症的反应等病症中基因表达发生的变化的常用方法，内华达州大学的平滑肌生物学小组已经开创了使用细胞-细胞分类技术来分选平滑肌组织的细胞成分的技术。特异性报告基因和表面抗原，然后进行细胞特异性转录组的全基因组评估。正在使用Fluidigm Biomark HD技术评估特定细胞类型中的高度突出基因，以确定随着平滑肌组织在发育或疾病状态中发生改变，基因表达的细胞间定量变化。转录分析还伴随着使用核糖体分析技术评估哪些转录本被积极翻译。我们也在创造特定基因被上调或下调的细胞系。COBRE的专题重点服务将由核心B（流式细胞仪/流式细胞术共享资源实验室）和核心C（单细胞分子表达）提供。提供详细的计划，以提高整个大学这些核心的用户群，并为国家的研究界创造长期可持续的核心设施。第三阶段将通过试点补助金计划进行补充，该计划旨在增加大学内多个项目对核心的使用。试点赠款将授予合格的调查人员，以获得使用核心技术的试点数据，以便利用核心技术提高赠款提案的力度，并在新的赠款获得资助时增加核心技术的使用。第三阶段由一个行政核心（核心A）领导，该核心通过主要研究者和项目协调员提供领导，通过内部指导委员会进行质量控制，并通过评估协调员和外部咨询委员会进行年度评估。

DESCRIPTION (provided by applicant): In this Phase III COBRE we will continue to develop the thematic focus: Smooth Muscle Plasticity, by continuing and enhancing Core laboratory services developed during COBRE Phases l&ll. Smooth muscles are complex tissues containing multiple cell types. Treating these muscles as homogenous tissues has been a common way to evaluate gene expression and the changes that occur in gene expression in conditions such as development and aging, diabetes, remodeling, responses to inflammation, etc. The smooth muscle biology group at the University of Nevada has pioneered techniques to sort the cellular components of smooth muscle tissues using cell-specific reporters and surface antigens and then to perform genome wide evaluations of cell-specific transcriptomes. Highly prominent genes in specific cell types are being evaluated with the Fluidigm Biomark HD technology to determine cell-by-cell quantitative changes in gene expression as smooth muscle tissues are altered in development or disease states. Transciptional analysis is also accompanied by evaluation of which transcripts are actively translated using Ribosome profiling techniques. We are also creating cell lines in which specific genes are up or down regulated. Services to the thematic focus of the COBRE will be provided by Core B (FACS/flow cytometry Shared Resource Lab) and Core C (Single Cell Molecular Expression). Detailed plans are provided to enhance the user base for these Cores throughout the University and to create long-lasting sustainable Core facilities for the research community in the State. The Phase III will be supplement by a Pilot Grant Program that is designed to increase usage of the Cores by several more projects within the University. Pilot Grants will be awarded to qualified investigators to obtain pilot data using Core technologies such that utilization of the Cores will enhance the strength of grant proposals and increase Core usage when new grants are funded. The Phase III is lead by an Administrative Core (Core A) which provides leadership via the Principal Investigator and Program Coordinator, quality control via an Internal Steering Committee, and annual evaluation through an Assessment Coordinator and an External Advisory Committee.