A new hemagglutination method for blood grouping

描述（由申请人提供）：拟议研究的长期目标是开发一种新型定量颗粒凝集技术，以提高血型分析的性能。传统的血凝血型检测方法存在灵敏度低、孵育时间长、主观性强、试剂用量大等缺点。正是在这种情况下，我们提出开发一种新的技术，它是从传统的血凝试验的上述限制自由。提出的免疫测定是一种灵敏和快速的方法，利用廉价的仪器。 第二阶段的具体目标是：1）进一步探索影响绩效的所有变量， 2）设计并组装一种可同时进行多种血型检测的设备。在最终的预生产原型中实施之前，Rototype旨在测试系统的每个技术元素的设计; 3）验证用于确定血型的检测分析（包括弱D群和C、c、E、e和K抗原）和预期抗体（反向分型）;检查每项试验的统计学显著数量的临床样本，并进行统计学分析，证明所得结果的重现性;比较传统和新开发检测试剂盒的性能。 值得注意的是，新方法提出了一种增强和检测不同来源的颗粒凝集的通用方法，包括乳胶、硅微粒和生物细胞。

DESCRIPTION (provided by applicant): The long-term goal of the proposed research is to develop a novel quantitative particle agglutination technology for improving the performance of blood typing assays. The conventional hemagglutination blood typing assays have serious drawbacks such as limited sensitivity, long time of incubation, subjectivity and large amount of reagents. It is in this context that we propose to develop a new technology, which is free from the aforementioned limitations of the conventional hemagglutination assays. The proposed immunoassay is a sensitive and rapid method that utilizes inexpensive instrumentation. The Specific Aims of Phase II are: 1) To further explore all variables affecting the performance of the new blood typing detection assays and optimize the performance of the assay; 2) To design and assemble an �rototype of the device for performing of several blood typing assays simultaneously. The �rototype is intended to test the design of every technical element of the system before it is implemented in the final pre- production prototype; 3) To validate the detection assays for determination of blood groups (including weak D group and C, c, E, e and K antigens) and expected antibodies (reverse typing); To examine a statistically significant number of clinical samples for each assay, and carry out statistical analysis and demonstrate reproducibility of the obtained results; To compare performance of the conventional and newly developed assays. Significantly, the new method presents a general approach for enhancement and detection of agglutination of particles of different origin, including latex, silic microparticles, and biological cells.