Progenitor Cell Analysis Core

项目摘要(参见说明)：为了推进中心和研究所的研究(以及中心的持续发展)，我们的祖细胞分析核心在三个相关领域提供专家服务、培训、仪器和资源：i)分析流式细胞术，以及FACS和MACS细胞分离服务；ii)高通量克隆/细胞成像、荧光测量和发光测量；iii)干细胞和祖细胞培养服务/资源，包括独特的细胞群体、外加ES细胞和经批准的细胞系。几项核心支持服务(例如，流式细胞仪和FACS)是不可或缺的独特组件，并被中心和研究所的研究人员广泛使用。BD FacsCalibur流式细胞仪和FACSAria-2细胞分选仪(近紫外光激光)等组合仪器可实现复杂的四色多参数分析。这包括细胞表面标记，以及(共同)分析细胞周期参数、细胞凋亡、磷蛋白信号、ROS、钙通量和许多其他信息的细胞特性。我们的专家人员还擅长浓缩和分离稀有细胞亚群(例如，使用MACS和FACS相结合的方法)。此外，该核心还支持祖细胞群体的基本分析(和操作)(Vicell系统、可注射多孔发光计、荧光计、电穿孔/转基因单元)。最近，我们还推出了几种创新的基于细胞的分析，例如通过STEMvision进行细胞和菌落扫描。关于干细胞和祖细胞培养和操作的服务和资源，这些服务和资源进一步受益于专门的组织培养设施(蔡司和EVOS荧光显微镜系统、细胞培养罩和孵化器、缺氧室、临床离心机、专用试剂)。我们的前体细胞分析核心还与组织病理学、分子表型、共聚焦显微镜和病毒载体方面的中心和研究所核心直接互动；促进多单位的有效互动；在新的中心和研究所教师招聘中发挥重要作用；并继续推进我们的生物医学研究调查(以及国家竞争性拨款申请)。

PROJECT SUMMARY (See Instructions): Towards advancing Center and Institute investigations (and continued Center development), our Progenitor Cell Analysis Core provides expert services, training, instrumentation and resources in three related areas: i) analytical flow cytometry, together with FACS and MACS cell isolation services; ii) high through-put colony/cell imaging, fluorimetry, and luminometry; iii) services/resources for stem & progenitor cell culture, including unique cell populations, plus ES cells and approved cell lines. Several core supported services (eg, flow cytometry and FACS) are indispensable, unique components, and are used widely by Center and Institute investigators. Combined instrumentation as a BD FacsCalibur flow cytometer plus FACSAria-2 cell sorter (near-UV laser) enable complex four color multi-parameter analyses. This includes cell surface markers, as well as (co-) analyses of cell cycle parameters, apoptosis, phosphoprotein signaling, ROS, calcium flux and a number of additional informative cell properties. Our expert staff are also skilled in the enrichment and isolation of rare cell sub-populations (eg, using combined MACS plus FACS approaches). In addition, this core supports basic analyses (and manipulations of) progenitor cell populations (Vicell system, injectable multi-well luminometer, fluorimeter, electroporation/transfection units). Recently, we also have launched several innovative cell-based analyses, with cell and colony scanning via STEMvision as one example. With regards to services and resources for the culture and manipulation of stem and progenitor cells, these benefit further from a dedicated tissue culture facility (Zeiss and Evos fluorescent microscopy systems, cell culture hoods and incubators, hypoxia chambers, clinical centrifuges, specialty reagents). Our Progenitor Cell Analysis Core also interacts directly with Center and Institute cores in Histopathology, Molecular Phenotyping, Confocal Microscopy and Viral Vectors; facilitates productive multi-unit interactions; provides vital roles in new Center and Institute faculty recruitment; and continues to advance our biomedical research investigations (together with nationally competitive grant applications).