Study of Accessory Lacrimal Gland and Precursor Cell Biology

项目摘要 这是一份K 08指导临床科学家职业发展奖的提案，旨在培训Vinay Kumar博士 阿卡鲁本应用程序的主要目标是作为培训Aakalu博士关键技能的基础 有必要发展成为一个独立的科学研究者专注于泪细胞和前体细胞 生物学拟议的教育计划的具体目标是：1）研究生水平的细胞， 分子，干细胞生物学和基本的翻译/临床试验课程工作在伊利诺伊大学， 芝加哥2）在的指导下进行副泪腺细胞和干细胞生物学的基础科学研究 一个经验丰富的四人指导委员会3）研讨会和会议的参与大学 伊利诺伊州和伊利诺伊大学以外。指导委员会由干细胞专家组成， 眼科、分子细胞生物学和转化研究。每位导师在培训临床医生方面经验丰富- 科学家指导委员会将专注于指导Aakalu博士的研究发展 技能，分析思维和基础和转化研究的道德表现。总体 该提案的研究目标是更好地了解副泪腺细胞生物学， 从这个组织中识别和分离前体细胞。该项目的具体目标是：1.开发一个 更好地了解ALG的分布、生长要求以及随患者特征的变化。1a.一 将构建尸体人眼眶的三维图以确定ALG的位置 作为合理采集决策的基础。1b.表征ALG组织， 人类手术标本，并将这些组织中的稳健性或组织病理学变化与相关 患者特征（年龄、性别、种族、激素状态）。1c.从人外科手术中开发ALG培养物 标本，以更好地了解细胞存活和扩增的要求。2.建立…的存在 ALG组织中的前体细胞，并分离和表征这些前体细胞。2a.识别前兆标志物 阳性ALG细胞，并使用人类样本确定特征标志物模式， 组织微阵列上的免疫组织化学。2b.利用荧光细胞分离前体候选细胞 分选并确定在不同患者中是否注意到前体候选细胞群的变化 特色2c.确定分离的前体候选细胞是否具有干细胞特性。2d.建立 从前体候选人培养，并确定这些培养物是否可以产生功能性ALG细胞。

Project Abstract This is a proposal for a K08 Mentored Clinical Scientist Career Development Award to train Dr. Vinay Kumar Aakalu. The primary goal of this application is to serve as a basis for training Dr. Aakalu in the critical skills necessary to develop into an independent scientific investigator focused on lacrimal cell and precursor cell biology. The specific aims of the proposed educational program are the performance of 1) graduate level cell, molecular, stem cell biology and basic translational/clinical trial course work at the University of Illinois at Chicago 2) basic scientific investigations in accessory lacrimal cell and stem cell biology under the guidance of an experienced four person mentoring committee 3) seminar and conference participation at the University of Illinois and outside the University of Illinois. The mentoring committee is made up of experts in stem cell, ocular, molecular cell biology and translational research. Each mentor is well experienced in training clinician- scientists. The mentoring committee will be focused on guiding Dr. Aakalu in the development of research skills, analytical thinking and the ethical performance of basic and translational research. The overarching research goals of the proposal are to develop a better understanding of accessory lacrimal cell biology and to identify and isolate precursor cells from this tissue. The specific aims of the project are to: 1. Develop a better understanding of ALG distribution, growth requirements, and changes with patient characteristics. 1a. A three dimensional map of the cadaveric human orbit will be constructed to determine the location of ALG tissue throughout the ocular surface as a basis for rational harvesting decisions. 1b. Characterize ALG tissue in human surgical specimens and relate the robustness or histopathologic changes in these tissues to relevant patient characteristics (age, gender, race, hormonal status). 1c. Develop ALG cultures from human surgical specimens to better understand requirements for cell survival and expansion. 2. Establish the presence of precursor cells in ALG tissue and isolate and characterize these precursor cells. 2a. Identify precursor marker positive ALG cells and determine a signature marker pattern using human specimens and immunohistochemistry on tissue microarrays. 2b. Isolate precursor candidate cells utilizing fluorescence cell sorting and determine if changes in precursor candidate cell populations are noted with different patient characteristics. 2c. Determine if precursor candidate cells isolated have stem cell properties. 2d. Establish cultures from precursor candidates and determine if these cultures can produce functional ALG cells.