IGF::OT::IGF A MULTIFUNCTIONAL, REUSABLE AND AUTOMATED MICROFLUIDIC SMART PLATE FOR HIGH THROUGHPUT LIVE CELL ASSAYS

本项目旨在设计和开发一种多功能、可重复使用和自动化的微流控智能板，用于高通量、活细胞分析。该系统将微流控多孔室阵列和环境测量系统结合到一个设备中，实现对实验环境因素的高精度和闭环控制。现有的环境控制室很大，物理上很笨重，获得起来也很昂贵，而且是为单一的显微镜类型和配置而设计的。我们的设备是活细胞延时和环境响应研究的独特解决方案。只需一台设备，研究人员就可以注入刺激，监测和改变环境因素，捕捉高数值孔径图像，并测量汇流。该设备将利用普遍可用的部件和制造方法，使从研究实验室到工业制造商的广泛感兴趣的各方能够复制。

This project aims to design and develop a multifunctional, reusable and automated microfluidic smart-plate for high throughput, live cell assays. This system will combine a microfluidic multi-well chamber array and an environmental measurement system into a single device, enabling high accuracy and closed-loop control over experiment environmental factors. Existing environmental control chambers are large, physically cumbersome and expensive to obtain, while being designed for a single microscope type and configuration. Our device is a singular solution for live cell time-lapse and environmental response studies. With a single device, researchers can inject stimuli, monitor and alter environmental factors, capture high numerical aperture images, and measure confluence. The device will make use of commonly available components and manufacturing methods, enabling replication by a wide range of interested parties, from research labs to industry manufacturers.