Pixel Array Detector for Macromolecular Crystallography

 描述(由申请人提供)：东北协作访问团队(NE-CAT)在高级光子源(APS)的24区操作两条用于大分子结晶学的波荡器光束线。光束线24-ID-C配备了Dectris Pilatus 6MF像素阵列探测器(PAD)，光束线24-ID-E配备了基于ADSC Q315 CCD的探测器。NE-CAT已经运营了15年，目前超额认购。超过85%的束流时间被分配给NIH资助的研究项目。在过去的12个月里，160多份出版物是根据东北-CAT收集的数据出版的。PADS在不到五年前才开始商业化，现在已被确定为在同步加速器光束线上收集大分子结晶学数据的最佳解决方案。我们的Pilatus 6MF大约在三年前投入使用，它的推出彻底改变了NE-CAT的数据收集。与基于CCD的探测器相比，PAD具有无读出噪声、更高的动态范围和更高的读出速度。这些功能允许无快门数据收集和嵌入不透明冷冻介质中的小晶体的衍射对准，并导致数据质量和分辨率的提高。因此，我们能够在生产力相应提高的情况下满足更多用户的需求。与2013年相比，2014年BEAM LINE 24-ID-C的出版物数量增加了20%以上。我们使用Pilatus 6MF的经验、我们的ADSC Q315 CCD探测器不再受支持的老化、我们的24-ID-E X射线光学系统的升级以在样品处产生直径为5微米的强X射线束，以及计划中的APS晶格升级，这些都促使我们为世代垫Dectris Eiger 16M申请资金。Eiger 16M将安装在NE-CAT光束线24-ID-E上，作为Q315的替代品。所有必要的基础设施都已就位，包括384至强核心计算集群和432 TB的磁盘存储。Eiger 16M具有更小的像素(75微米，而Pilatus 6MF为150微米)和更快的成帧速率。Eiger 16M提供了Pilatus 6MF的所有优势以及更多优势。特别是，Eiger 16M更适合微束结晶学，这是我们的主要重点领域之一。这些优点将使我们能够开发系列微囊造影术，其中使用非常强的光束来收集微晶体中的一帧或几帧。然后对这些帧进行处理和合并，以创建一个完整的数据集。从长远来看，目前安装在24-ID-E的二次KB聚焦系统与APS多弯消色差晶格升级相结合，将使光束亮度增加约两个数量级。这与我们未来的微束结晶学计划非常匹配。Eiger 16M将进一步增强我们在广泛项目中的技术能力，其中许多项目需要Eiger 16M前所未有的特性。

 DESCRIPTION (provided by applicant): The Northeastern Collaborative Access Team (NE-CAT) operates two undulator beamlines for macromolecular crystallography at Sector 24 of the Advanced Photon Source (APS). Beamline 24-ID-C is equipped with a Dectris PILATUS 6MF pixel array detector (PAD) and beamline 24-ID-E is equipped with an ADSC Q315 CCD-based detector. NE-CAT has operated for 15 years and is currently oversubscribed. More than 85% of the beam time is allocated to NIH funded research projects. During the past 12 months, over 160 publications resulted from data collected at NE-CAT. PADs became commercially available less than five years ago and are now established as the best available solution for collecting macromolecular crystallography data at synchrotron beamlines. Our PILATUS 6MF was commissioned about three years ago and its availability has revolutionized data collection at NE-CAT. Compared to CCD-based detectors, PADs have no readout noise, higher dynamic range, and much higher readout speed. These features allow shutterless data collection and diffractive alignment of small crystals embedded in opaque cryomedia, and result in improved data quality and resolution. Consequently, we are able to accommodate more users with a commensurate increase in productivity. The number of publications from beamline 24-ID-C in 2014 increased by more than 20% compared to the number of publications in 2013. Our experience with the PILATUS 6MF, the aging of our ADSC Q315 CCD detector, which is no longer supported, an upgrade of our 24-ID-E X-ray optics to produce intense 5 µm diameter X-ray beams at the sample, and the planned APS lattice upgrade, has led us to request funding for the generation PAD, the Dectris EIGER 16M. The EIGER 16M will be installed at the NE-CAT beamline 24-ID-E as a replacement for the Q315. All of the necessary infrastructure is in place including a 384 Xeon core compute cluster and 432 TB of disk storage. The EIGER 16M has smaller pixels (75 µm compared to 150 µm for the PILATUS 6MF) and a faster framing rate. The EIGER 16M provides all of the advantages of the PILATUS 6MF and more. In particular, the EIGER 16M is better suited for microbeam crystallography, which is one of our major areas of emphasis. These advantages will allow us to develop serial microcystallography in which very intense beams are used to collect one or a few frames from microcrystals. The frames are then processed and merged to create a complete dataset. In the long term, the secondary KB focusing system currently being installed at 24-ID-E in combination with the APS multi-bend achromat lattice upgrade will provide about two orders increase in beam brilliance. This is ideally matched to our future plans for our microbeam crystallography program. The EIGER 16M will further enhance our technological capabilities for a broad range of projects, many of which require the unprecedented characteristics of the EIGER 16M.