Mouse Genetics Core

摘要 衰老和基因工程小鼠模型的开发和利用对理解 特定基因在年龄相关性耳聋(ARHL)中的作用我们将要求特定品系的老化(衰老) 在NIA支持的NIH设施中生产的商业上无法获得的小鼠， Http://www.nia.nih.gov/research/dab/aged-rodent-colonies-handbook.中的所有项目和核心C 提出的PPG广泛利用了这些衰老的小鼠，并利用各种遗传方法来研究 几个基因在内耳和听觉神经通路中的作用。事实上，拟议的PPG将需要 在老化的听觉系统中使用导致听力损失的疾病模型。鼠标的用途 核心是为品系特异性老龄小鼠群体的建立和世代提供服务 基因靶向和转基因小鼠的管理。创始老鼠将被识别、饲养和维护在 UNR的动物设施。此外，还将培育和老化几个小鼠品系，并提供给实验 在所有三个项目和核心C中提出。核心还将负责维护和分发 鼠标线指向每个单独的项目。这将提供最具成本效益的共享和利用 老鼠模型。此外，核心还将冻结存储关键线路，以防意外丢失。最后， 该中心将为其他对急性淋巴细胞性白血病小鼠模型感兴趣的实验室提供资源。

Abstract Development and utilization of aged and genetically engineered mouse models are crucial to understanding the roles of specific genes in age-related hearing loss (ARHL). We will require strain-specific aged (senescent) mice that are not available commercially and are produced at the NIA-supported facility at the NIH, http://www.nia.nih.gov/research/dab/aged-rodent-colonies-handbook. All projects and Core C within the proposed PPG makes extensive use of these aged mice, and utilize a variety of genetic approaches to study the role of several genes in the inner ear and auditory neural pathways. Indeed, the proposed PPG will require the use of diseased models that result in hearing loss in the aging auditory system. The purpose of the mouse core is to provide services for the establishment of a strain-specific aged mouse colony and the generation and management of gene-targeted and transgenic mice. Founder mice will be identified, bred, and maintained in an animal facility at UNR. Additionally, several mouse lines will be bred and aged and supplied for experiments proposed in all three projects and Core C. The Core will also be responsible for maintaining and distributing mouse lines to each individual project. This will provide the most cost effective sharing and utilization of the mouse models. In addition, the Core will also cryopreserve critical lines in case of an inadvertent loss. Finally, the Core will be a resource for other laboratories interested in mouse models of ARHL.