National Xenopus Resource Center

 描述(由申请者提供)：总体内容本项目支持继续为位于马萨诸塞州伍兹霍尔海洋生物实验室的国家非洲爪哇资源(NXR)提供资金，该资源被确定为非洲爪哇社区的两个最优先事项之一。NXR成立于2010年，是为研究非洲爪哇两栖动物模型系统的研究人员提供的国家资源，其中包括两个物种非洲爪哇和热带非洲爪哇。NXR是一个社区动物种群的国家储存库，也是一个高级培训场所，极大地促进了大众的生产力 研究人员。它是一个面向社区的资源中心，为所有非洲爪哇研究人员提供服务，包括从以研究为重点的大型大学到小型文理学院的个人。随着生物学研究变得越来越复杂，往往需要专门的动物品系，涉及不同的技术，单个实验室单位不足以满足解决重大生物学问题所需的所有需求，集中的储存库或库存中心变得必不可少。这笔赠款有五个主要目标。首先，NXR将维持现有的青蛙种群，并获得新的品系，使其可供社区使用；这包括两个物种的特殊近交系、野生型和突变品系。在第二个目标中，我们概述了NXR定制的研究服务，用于创建提供给研究社区的转基因和突变动物。在第三个目标中，我们概述了我们为非洲爪哇社区创建的资源，这些资源有助于促进社区中新技术的使用。这些资源得益于用于研究、教学和住房的一流的MBL设施。这包括发展由每个领域的专家讲授的新的高级培训讲习班，用于教授和传播专门技术；这包括关于生物信息学、成像、基因组编辑、转基因和畜牧业的讲习班。在这一目标中，我们还概述了研究型酒店服务，它为研究人员提供来MBL进行短期访问和利用NXR资源的机会。最后两个目标集中在应用研究上。在目标4中，我们描述了新的转基因技术和新的基因组编辑技术的增强。在最后一个目标中，我们建议优化莱氏X.laevis精子的冷冻保存，并开发一种新的健康筛查PCR服务来测试特定病原体的存在。

 DESCRIPTION (provided by applicant): Overall Component This project supports continued funding for the National Xenopus Resource (NXR) located at the Marine Biological Laboratory in Woods Hole, MA and identified as one of two top priorities for the Xenopus community. The NXR was established in 2010 to serve as a national resource for researchers working with the Xenopus amphibian model system, which includes two species Xenopus laevis and Xenopus tropicalis. The NXR serves as a national repository for community-generated animal stocks as well as serving as an advanced training venue that greatly facilitates productivity for a multitude of researchers. It is a community-oriented resource center that serves all Xenopus researchers, including individuals from large research-focused universities to small liberal arts colleges. As biological research becomes ever more complex, often requiring specialized animal lines and involving diverse technologies, individual laboratory units become insufficient to meet all of the demands required for addressing significant biological problems and a centralized repository or stock center becomes essential. There are five main aims to this grant. First, the NXR will maintain current stocks of frogs as well as obtain new lines making them available to the community; this includes special inbred, wild type and mutant lines of both species. In the second aim we outline the NXR custom research services for creating transgenic and mutant animals that are offered to the research community. In the third aim we outline the resources that we have created for the Xenopus community that helps to facilitate the use of new techniques in the community. These resources benefit from the excellent MBL facilities for research, teaching and housing. This includes the development of new advanced training workshops taught by experts in each field that serve to teach and propagate specialized techniques; this includes workshops on bioinformatics, imaging, genome editing, transgenesis and husbandry. In this aim we also outline the research hotel service, which provides opportunities for researchers to come to the MBL for short-term visits and use NXR resources. The last two aims are focused on applied research. In Aim 4 we describe new transgenic technology and enhancement of the new genome editing technology. In the last aim we propose to optimize sperm cryopreservation in X. laevis and develop a new health screening PCR service to test for the presence of specific pathogens.