Trace Elements (TE)

项目概要/摘要：微量元素（TE）共享资源 微量元素（TE）共享资源是专门用于测定微量元素的专用工具 浓度和形式的微量元素，在各种各样的生物和环境介质。的 无机元素化学与癌症的研究密不可分。无论是决定 和已知致癌物（如砷）的影响，元素（如 硒），或铂或铁纳米颗粒的治疗效果，显然需要常规的先进的 微量元素分析方法，以支持癌症研究。这些服务的关键性质， Norris Cotton癌症中心（NCCC）的研究计划已导致TE被指定为NCCC支持的 共享资源和未来CCSG资金的请求。在TE核心中，电感耦合等离子体质量 已经开发了光谱法（ICP-MS）方法来提供固体中痕量金属的分析， 溶液血液和脚趾甲TE服务包括溶液中的砷形态分析、 溶液和ICP-MS调谐和操作进行常规分析。有一些值得注意的癌症例子 利用TE资源进行研究。例如，TE核心开发了用于确定 大米、果汁和幼儿配方奶粉中的砷。此外，TE开发的空间分析显示， 组织切片内的微量元素，其以预定分辨率“原位”测定， 2-使用激光烧蚀-ICP-MS从生物薄切片获得三维元素图像图。 技术，TE核心开发了检测大鼠脑切片中Fe，Cu和Zn的方法。分析 专业知识由TE核心总监和一名博士级科学家和一名高级 研究技术员。TE核心与NCCC研究人员在5个NCCC计划中合作 （癌症流行病学，癌症机制，分子治疗学，癌症成像和放射生物学，以及 免疫学和癌症免疫治疗），以确定生物体内微量元素的浓度或形式 样品

Project Summary/Abstract: Trace Elements (TE) Shared Resource The Trace Element (TE) Shared Resource is a specialized facility dedicated to determining trace element concentration, and form of trace elements, in a wide variety of biological and environmental media. The chemistry of inorganic elements inextricably is linked with the study of cancer. Whether determining the fate and effects of known carcinogens (such as arsenic), the potential antagonistic effects of elements (such as selenium), or the therapeutic effects of platinum or Fe nanoparticles, there is a clear need for routine advanced trace element analytical methods to support cancer research. The critical nature of these services for the Norris Cotton Cancer Center (NCCC) research program has led to designation of TE as an NCCC-supported Shared Resource and to a request for future CCSG funding. In the TE core, Inductively Coupled Plasma Mass Spectrometry (ICP-MS) methodologies have been developed to provide analysis of trace metals in solids, solution, blood, and toenails. TE services include arsenic speciation in solutions, mercury speciation in solution, and ICP-MS tuning and operation for routine analysis. There have been notable examples of cancer research using TE resources. For example, the TE core developed innovative methods for determining Arsenic species in rice, juice, and toddler formulae. Also TE-developed spatial analyses show the distribution of a trace element within tissue sections, which is determined 'in situ' at pre-determined resolution, creating a 2-dimensional elemental image map from biological thin sections using laser ablation-ICP-MS. Using this technology, the TE core developed methods for detecting Fe, Cu, and Zn in rat brain sections. Analytical expertise is provided by the TE Core Director and a staff consisting of a PhD level scientist and an advanced Research Technician. The TE core has collaborated with NCCC researchers in 5 of the NCCC Programs (Cancer Epidemiology, Cancer Mechanisms, Molecular Therapeutics, Cancer Imaging & Radiobiology, and Immunology & Cancer Immunotherapy) to determine the concentration or form of trace elements in biological samples.