Chemical and biological diversity of arsenic compounds derived from marine products: Elucidation of their pharmacokinetics and metabolic activation

从对孟加拉国砷污染地区个人尿液样本的砷形态分析结果来看，无机砷（iAs）暴露的最佳生物标志物是尿液iAs和一甲基胂酸（MMA(V)）浓度。为了评估膳食砷的风险，我们进行了裙带菜摄食试验。检测到的尿砷种类有二甲基胂酸（DMA(V)）、氧基二甲基胂酸乙醇、氧基二甲基胂酸乙酸酯以及一些未识别的种类。我们开发并验证了气相色谱-质谱法测定尿液中的砷。采用配备ODS色谱柱的HPLC-TOF-MS和GC-FPD分析表明，二甲基一硫代胂酸（DMMTA(V)）与谷胱甘肽在体外反应生成二甲基一硫代胂酸-谷胱甘肽（DMMTA(V)-谷胱甘肽）配合物产生硫化氢。

From the results of arsenic speciation analysis of urine samples from individuals living in an arsenic-contaminated area in Bangladesh, the best biomarker of inorganic arsenics (iAs) exposure is urinary iAs and monomethylarsonic acid (MMA(V)) concentration. To evaluate the risk of dietary arsenic, we conducted wakame seaweed ingestion experiment. The detected urinary arsenic species were dimethylarsinic acid (DMA(V)), oxo-dimethylarsinoylethanol, oxo-dimethylarsinoylacetate, and some unidentified species. We developed and validated a GC-MS method to determine arsenic in urine.Analytical examination using HPLC-TOF-MS equipped with ODS column and GC-FPD showed that an in vitro reaction between dimethylmonothioarsinic acid (DMMTA(V)) and GSH produced hydrogen sulfide via the generation of dimethylmonothioarsinic acid-GSH (DMMTA(V)-GSH) complex.