Resource for Pre-Clinical Studies of AKI (Animal Models/lmaging/Renal Physiology

人类疾病的小鼠模型为肾脏病理生理过程提供了重要的见解，是测试急性肾损伤（AKI）治疗和预防方法的重要临床前资源。Core B的具体目的是提供设备和必要的技能1)研究AKI的小鼠模型，2)小动物成像，3)确定AKI的肾脏生理变化。该核心将专门提供(i)在开发和培训使用AKI啮齿动物模型方面的专业知识，特别是在缺血/再灌注损伤、败血症和肾移植的情况下，（ii）一个多模态小动物成像核心，将提供最先进的分子成像，包括功能、结构和代谢成像，使用磁共振成像/光谱、高频超声、微CT、伽马射线成像(伽马相机、微spect /CT、显微pet /CT)和光学成像（生物发光和荧光），以及（iii）生理学核心，将为全肾和单肾元水平的肾功能研究提供专业知识和培训，包括微穿刺技术和GFR的测定，管状液体和管状重吸收的微分析，肾血流动力学与小管肾小球反馈的评估，以及啮齿动物肾脏耗氧量的代谢评估。核心B还将为分离培养的啮齿动物的原代肾细胞和血管细胞提供技术专长。

Mouse models of human disease have provided essential insights into renal pathophysiological processes and are an important preclinical resource to test therapeutic and preventive approaches in acute kidney injury (AKI). The specific aims of Core B are to provide the facilities and requisite skills 1) to study murine models of AKI, 2) for small animal imaging, and 3) to determine renal physiological changes in AKI. This core will specifically provide (i) expertise in development and training in the use of rodent models of AKI specifically in the setting of ischemia/reperfusion injury, sepsis and renal transplantation, (ii) a multi-modality small animal imaging core that will provide state-of-the-art molecular imaging, including functional, structural and metabolic imaging using magnetic resonance imaging/spectroscopy, high frequency ultrasonography, microCT, gamma-ray imaging (gamma camera, microSPECT/CT, microPET/CT), and optical imaging (bioluminescence and fluorescence), and (iii) a physiology core that will provide expertise and training for studying renal function on the whole kidney and at the single nephron level, including micropuncture techniques and determination of GFR, microanalysis of tubular fluid and tubular reabsorption, renal hemodynamics with assessment of tubuloglomerular feedback, and metabolic assessment of kidney oxygen consumption in rodents. Core B will also provide technical expertise for the isolation of primary renal and vascular cells in culture from rodents. The intent of Core B is to provide unique resources that help overcome barriers for investigators to utilize relevant rodent models for in vivo studies and rodent cell lines for in vitro studies to advance understanding of the pathophysiology of AKI. Core B has been very successful in supporting the kidney research community. Since the inception of Core B, more than 5,800 procedures have been performed for 80 principal investigators involving 92 projects. Of the 80 investigators, 60 (75%) were non-core personnel. The number of investigators using Core B each year is increasing, as is the annual publication rate. Core B has also supported the research efforts of 11 Pilot and Feasibility grant awardees. These combined efforts have been currently recognized in 83 peer-reviewed publications. The sophisticated infrastructure coupled with the unique expertise of Core B will continue to catalyze collaborative efforts between and UCSD and produce innovative new initiatives that will advance AKI research.