Workshop on Chromatin Interaction Analysis using Paired-End Tag Sequencing

 描述(由申请人提供)：揭示调节区和基因编码区之间的相互作用对于理解健康和疾病中基因调控的机制至关重要。染色质相互作用分析配对末端标签测序(CHIA-PET)为在全球范围内高分辨率和高特异性地定位染色质相互作用网络提供了一种新的策略。CHIA-PET分析产生的数据可用于绘制蛋白质因子结合位点的图谱，以及构建与特定蛋白质因子相关的全基因组染色质相互作用组图。拟议的“成对末端标签测序染色质相互作用分析研讨会”将培训年轻科学家(研究生、博士后实习生、新的研究人员)使用Chia-PET作为工具，询问染色质相互作用、3D基因组组织和人类健康和疾病中的基因组调控。我们为期五天的CHIA-PET研讨会提供了教学和基于实验室的教学的平衡，并有足够的机会在参与者和课程教师之间进行互动。参与者将进行CHIA-PET实验，从染色质免疫沉淀(CHIP)步骤到高通量测序，并通过生物信息学分析和数据处理阶段。2014年，日本宇宙航空研究开发机构成功试行了CHIA-PET讲习班，登记等候名单表明，对这一技术培训的需求很大。由美国国立卫生研究院共同基金于2015年发起的4D核组项目将进一步增加该领域的研究活动，并可能在CHIA-PET和相关技术方面产生显着的额外的Interet。拟议的课程将于每年深秋在康涅狄格州法明顿的杰克逊基因组医学实验室举行。讲座、讨论和实验室工作将在上午、下午和晚上进行，总共大约50个小时的授课和动手培训。杰克逊实验室是美国国家癌症研究所指定的基础研究癌症中心，在举办高级课程和研讨会方面有着悠久的历史。

 DESCRIPTION (provided by applicant): Uncovering the interplay between regulatory regions and gene coding regions is essential for understanding the mechanisms governing gene regulation in health and disease. Chromatin Interaction Analysis Using Paired-End Tag Sequencing (ChIA-PET) offers a new strategy for mapping chromatin interaction networks on a global scale with high resolution and specificity. Data generated from ChIA-PET analysis can be used to map protein-factor binding sites, as well as to construct genome-wide chromatin interactome maps associated with a specific protein factor of interest. The proposed "Workshop on Chromatin Interaction Analysis by Paired End- Tag Sequencing", will train young scientists (graduate students, post-doctoral trainees, new investigators) in the use of ChIA-PET as a tool for asking questions about chromatin interactions, 3D genome organization and genome regulation in human health and disease. Our five-day ChIA-PET workshop provides a balance of didactic and laboratory-based instruction with ample opportunities for interactions among participants and course faculty. Participants will conduct a ChIA-PET experiment from the chromatin immunoprecipitation (ChIP) step to high throughput sequencing and through the bioinformatics analysis and data processing phases. In 2014 JAX successfully piloted the ChIA-PET workshop and a registration waitlist demonstrated significant demand for this technical training. The 4D Nucleome project initiated by the NIH Common Fund in 2015 will further increase research activity within this field and will likely generate significant additional interet in ChIA-PET and related technologies. The proposed course will be held annually during the late fall at The Jackson Laboratory for Genomic Medicine in Farmington, Connecticut. Lectures, discussions, and laboratory work will be held morning, afternoon, and evening for a total of approximately 50 hours of didactic and hands-on training. The Jackson Laboratory is an NCI-designated Basic Research Cancer Center and has a long history of hosting advanced courses and workshops.