Enhancing Data Reproducibility through Cell Authentication Training

 描述（由申请人提供）：“通过细胞认证培训增强数据复制能力”旨在创建一个可导出的细胞认证在线培训模块，适用于所有在研究中使用细胞系的研究生研究人员，包括研究生，博士后学者和初级教师。NIH目前资助了大约9,000个涉及细胞系的项目和子项目，总支出为37亿美元。虽然国家癌症研究所资助了这些项目的大部分，但NIH和FDA的所有研究所都资助了使用细胞系的研究。细胞系污染和错误鉴定是研究再现性问题的主要原因。存在最佳做法来显著减少这些问题，并确保更有效地使用研究资金，但这些做法的认识和实施仍然很低。我们认为，有效的“主动学习”培训模式和强有力的宣传运动将提高认识和执行。该模块将包含高度互动的培训单元，包括“如何”视频，将学习转化为实践，让学员回到实验室练习他们的技能，并跟踪他们的培训是否被纳入他们未来的研究。我们将建立合作伙伴关系，并制定社交媒体策略，以传播和推广该模块给所有在工作中使用细胞系的研究生研究人员。该模块将进行优化，以供个人以“仅在线”格式直接访问材料，以及由负责任的研究行为和其他课堂环境中的学员使用“混合”格式，其中一些材料可以在线独立查看，而其他材料则由小组在课堂上使用。总教学时间为2-4小时。作为这种培训的结果，为什么和如何进行细胞认证的知识将得到提高，涉及细胞系的研究重现性问题将减少。该项目将节省数百万美元的研究费用，并减少从实验室到诊所再到床边的翻译时间。

 DESCRIPTION (provided by applicant): "Enhancing Data Reproducibility Through Cell Authentication Training" aims to create an exportable online training module on cell authentication suitable to all postgraduate researchers who use cell lines in their research, including graduate students, postdoctoral scholars, and junior faculty members. NIH currently funds approximately 9,000 projects and sub-projects involving cell lines, at a total spend of $3.7B. While the National Cancer Institute funds the bulk of these projects, all Institutes at the NIH and the FDA fund studies using cell lines. Cell line contamination and misidentification are major contributors to research reproducibility problems. Best practices exist to reduce these problems significantly and ensure more effective use of research dollars, and yet awareness and implementation of these practices remain low. We believe that an effective "active learning" training module and robust dissemination campaign will improve both awareness and implementation. This module will contain highly interactive training units including "how to" videos that will turn learning into practice by sending the trainees back into the laboratory to practice their skills, and tracking whether their training is incorporated into their future researh. We will build partnerships and develop social media strategies to disseminate and promote this module to all postgraduate researchers who use cell lines in their work. The module will be optimized for use both by individuals accessing the material directly in an "online-only" format, and by trainees within Responsible Conduct of Research and other classroom settings using a "blended" format in which some materials are viewed independently online and others are used by groups in class. The total instruction time will be 2-4 hours. As a result of this training, knowledge of why and how to perform cell authentication will improve and research reproducibility problems involving cell lines will decrease. This project will save millions of dollars in wasted research expenditures and decrease translation time from bench to clinic to bedside.