Pathology Core

总结-病理学核心 老年动物的病理学评估对于确定所观察到的变化是否为 动物年龄与潜在的病理状况相关或独立。因此， 以获得对衰老动物的准确和全面的病理学评估。病理学核心将发挥 在圣安东尼奥内森休克衰老中心的关键作用，因为病理学呈指数增长， 这是导致年龄增长的主要原因，也是与年龄有关的发病率和死亡率的主要原因。病理学知识 与用于研究衰老的干预措施相关的病变对于解释这些干预措施的影响至关重要。 对衰老过程的干预。这些知识也将提供深入了解的基础 干预措施效果背后的机制。病理学核心将建立在广泛的经验， 研究人员在圣安东尼奥和核心领导人的专业知识，在病理分析的实验 动物模型因此，病理学核心的具体目标是： 1.对已建立和新的癌症患者进行全面的临终和横断面病理分析， 啮齿动物模型以及用于衰老研究的其他物种，其自发死亡或在 老化动物模型和寿命评估核心维持的群体中的特定年龄点。 2.进行免疫组织化学，分子和定量形态学分析， 啮齿类动物模型和其他物种的组织/器官， 几种病变和年龄相关组织学变化的免疫组织化学分析和B）原位 进一步从分子水平分析组织学变化。 3.继续a）积累一个全面的组织病理学数据和图像数据库，作为一种资源 对于研究者的分析，B）为新研究提供基本病理学信息，以及c） 通过收集和储存组织样本（石蜡和冷冻块）作为资源，创建组织档案 用于分析所要求的样品和进行新的形态学研究。 4.为研究者提供组织病理学服务，准备石蜡和冷冻块， 未染色的载玻片和进行各种染色（包括特殊染色）。新服务包括（a） 制作用于组织学/形态学实验的组织阵列载玻片，以及（B）进行激光捕获 显微解剖以进行进一步的分子分析。 5.帮助对动物模型的基础生物学感兴趣的教师和学生进行病理学 资助申请和手稿准备所需的分析。

SUMMARY – PATHOLOGY CORE The pathological assessment of old animals is important to determine whether the changes observed as animals age are associated with or independent of underlying pathological conditions. It is, therefore, essential to obtain accurate and thorough pathological assessments of aging animals. The Pathology Core will play a key role in the San Antonio Nathan Shock Aging Center because pathology increases exponentially with advancing age and is largely responsible for age-related morbidity and mortality. Knowledge of the pathological lesions associated with interventions used to study aging is essential to interpreting the impact of these interventions on the aging process(es). This knowledge will also provide insight into the underlying mechanism(s) behind the interventions' effects. The Pathology Core will build on the extensive experience of researchers at San Antonio and the expertise of the Core Leaders in pathological analyses of experimental animal models. Thus, the Specific Aims of the Pathology Core are: 1. To conduct comprehensive end-of-life and cross-sectional pathological analyses of established and new rodent models as well as other species used in aging research that die spontaneously or are sacrificed at specific age points in colonies maintained by the Aging Animal Models and Longevity Assessment Core. 2. To conduct immunohistochemical, molecular, and quantitative morphometric analyses of the tissues/organs of rodent models and other species by performing a) morphometric and immunohistochemical analyses on several lesions and age-related histological changes and b) in situ hybridization to further analyze the histological changes at the molecular level. 3. To continue to a) accrue a comprehensive database of histopathological data and images as a resource for analyses by investigators, b) provide basic pathological information for new investigations, and c) create a tissue archive by collecting and storing tissue samples (paraffin and frozen blocks) as a resource for analyses of samples as requested and for new morphological research. 4. To provide histopathology services for investigators by preparing paraffin and frozen blocks, making unstained slides, and performing various staining (including special staining). New services include (a) making tissue array slides for histological/morphological experiments, and (b) performing laser capture microdissection for further molecular analyses. 5. To assist faculty and students interested in the basic biology of animal models with the pathological analyses needed for grant applications and manuscript preparation.