Core 4: Laboratory p354-364

项目主任/首席调查员(最后、第一、中间)：Khshnan-Sarin，Suchltra/O‘Malley，Stephanie S. 具体目标：该实验室核心的目的是支持在以下组织赞助下开展的研究 拟议的中心，通过提供对与选定烟草有关的接触的客观和定量估计 药物/化合物，由它们在生物体液中的浓度反映出来。与其中一个优先事项保持一致 在FOA中，拟议的项目关注的是通过更好地了解如何减少成瘾， 烟草产品含量的一些普遍变化可能会影响尼古丁成瘾的风险。 研究策略包括通过各种途径单独和与其他药物联合使用尼古丁 选定的添加剂，包括薄荷醇，可能会影响对尼古丁的反应。对研究的最佳解读 结果要求尼古丁和/或薄荷醇暴露在不同个体和实验对象之间的差异 武器是可以考虑的。最初的分析目标清单包括尼古丁、可替宁、反式3-羟基可替宁/可替宁 (OHC/C)比率、薄荷醇、葡萄糖醛酸苷和未结合薄荷醇(用于体外溶液)。然而， 根据中心不断发展的需求，实验室核心将有能力建立其他分析方法。

Program Director/Principal Investigator (Last, First, Middle): Khshnan-Sarin, Suchltra/O'Malley, Stephanie S. SPECIFIC AIMS: The purpose of this Laboratory Core is to support research carried out under the auspices of the proposed Center by providing objective and quantitative estimates of exposure to selected tobacco related drugs /compounds, as reflected by their concentrations in biological fluids. Consistent with one of the priorities in the FOA, the proposed projects are concerned with reducing addiction through better understanding of how , some prevalent modifications in the content of tobacco products may influence risk of addiction to nicotine. Research strategies include administration, by various routes, of nicotine alone, and in conjunction with other selected additives, including menthol, that may influence responses to nicotine. Optimal interpretation of study outcomes requires that variations in nicotine and/or menthol exposure across individuals and experimental arms be considered. The initial list of analyte targets includes nicotine, cotinine, trans 3-OH cotinine/cotinine (OHC/C) ratios, menthol glucuronide and unconjugated menthol (for in-vitro solutions). However, the Laboratory Core will have the ability to set up other assays as mandated by the evolving needs of the center...