Collaboratory for atlasing cell type anatomy in the female and male mouse brain

尽管一个多世纪以来，科学家们一直被脑细胞的多样性所吸引，但自从 圣地亚哥·拉蒙·卡哈尔的初步解剖学描述，直到最近，新的 科技让我们真正体会到哺乳动物大脑中细胞类型的惊人复杂性。 然而，即使在今天，我们对细胞类型解剖和功能的了解在很大程度上局限于少数几个大脑区域，如 如感觉皮质或海马体。这种全脑数据的缺乏导致了从一种 大脑区域连接到具有相似结构的其他区域。然而，我们的初步研究采用了定量的 全脑分析显示了出乎意料的区域特定细胞类型比率的差异，并发现 细胞解剖学中新的基于性别的差异可以帮助我们理解大脑发育， 接线和功能。因此，我们认为，弥合我们对细胞体系结构的认识上的差距 大脑将是实现对大脑作为一个系统的现代定量理解的主要贡献。 在这里，我们建议建立一个解剖学合作实验室，用于系统地绘制细胞类型分布的图谱 以及雌雄两脑的形态。该合作实验室将应用标准化、不偏不倚和大量 我们实验室开发的自动化方法来绘制&gt；80分子的分布和形态图谱 确定了女性和男性大脑所有区域的细胞类别和细胞类型。这项工作将产生最大的收益 到目前为止，哺乳动物大脑中细胞类型解剖的全面特征，建立了一个结构 建立一个完整的细胞类型脑图谱的基础。 具体目标1：量化并绘制分子定义的细胞类型的脑内分布图集。这 这项工作将生成分子定义的细胞类别和细胞分布的定量脑图谱 成人大脑共同协调框架(CCF)内的类型。 具体目标2：量化并绘制分子定义的细胞类型的全脑形态图谱。这 工作将产生一个全面的结构图谱的躯体树突状细胞类型的所有 女性和男性大脑中的CCF解剖区域。 具体目标3：开发和部署神经信息学基础设施：为 单元类型特定的数据、工具和分析。这项工作将通过CSHL细胞传播生成的数据 通过脑细胞数据中心(BCDC)和特定类型的BrainArchitecture Web门户。 好了！

Even though scientists have been captivated by the diversity of brain cells for well over a century, since the initial anatomical descriptions by Santiago Ramón y Cajal, it is only recently that the developments in new technologies allowed us to truly appreciate the astonishing complexity of cell types in the mammalian brain. Yet, even today, our knowledge of cell type anatomy and function is largely limited to a few brain areas, such as the sensory cortex or the hippocampus. This lack of whole-brain data has led to generalizations from one brain area to other regions with similar architecture. However, our preliminary studies applying quantitative whole brain analyses have revealed unexpected region specific differences in cell type ratios and uncovered new gender-based differences in cellular anatomy that can inform our understanding of brain development, wiring and function. We thus believe that closing the gaps in our knowledge of the cellular architecture of the brain will be a major contribution to enable a modern quantitative understanding of the brain as a system. Here we propose to establish an Anatomical Collaboratory for Systematic Atlasing of Cell-Type Distribution and Morphology in female and male brain. This Collaboratory will apply standardized, unbiased and largely automated methods developed in our laboratories to atlas the distribution and morphology of >80 molecularly defined cell classes and cell type across all regions of the female and male brain. This work will yield the most comprehensive characterization of cell type anatomy in the mammalian brain to date, establishing a structural basis for building an integrated Cell Type Brain Atlas. Specific Aim 1: Quantify and atlas the brainwide distribution of molecularly defined cell types. This work will generate a quantitative brain atlas of the distributions of molecularly defined cell classes and cell types within the Common Coordinated Framework (CCF) of the adult brain. Specific Aim 2: Quantify and atlas the brainwide morphologies of molecularly defined cell types. This work will generate a comprehensive structural brain atlas of somatodendritic cell-type morphologies across all CCF anatomical areas in the female and male brain. Specific Aim 3: Develop and deploy a neuroinformatics infrastructure: an integrated web portal for cell-type specific data, tools, and analytics. This work will disseminate the generated data via CSHL cell type-dedicated BrainArchitecture web portal and through the BRAIN Cell Data Center (BCDC). !