Gene Analysis Shared Resource

基因分析共享资源(GASR)为梅奥临床癌症研究人员提供高效、高质量、 以经济高效的方式获得最先进的基因组学技术，以研究基因结构和 功能。自2003年以来，GASR各组成部分一直以协调一致的方式工作，以提供 在核酸测序、基因表达方面的技术专长、仪器设备和协调支持 侧写和基因分型。通过RNAi支持NextGen测序和基因表达调控 分析是在2007年添加的。该设施提供的服务包括核糖核酸的深度测序研究 和DNA在Illumina HiSeq和MiSeq仪器上，以及太平洋生物科学单分子实时 定序器。此外，我们还提供使用ABI 3730x1测序仪的全方位测序服务，以及全面的 利用Affymetrix基因芯片™技术和灯盏花珠芯片进行基因分型和基因转录本分析。 甲基化分析使用Illumina甲基化阵列和简化的代表性亚硫酸氢盐 Illumina HiSeq仪器的测序。RNA提供了一种重要的附加功能 干扰共享资源，为慢病毒包装试剂提供特殊合同定价 生产和使用完整的开放生物系统pGIPZ基因敲除文库，供所有梅奥研究人员使用。 四项活动每一项都由一名在重点领域具有专门知识的高级工作人员级联合主任领导。 在重要的机构支持下，我们继续在#年增加新的工具、人员和服务 支持癌症中心的活动。有一个发达的合作管理结构， 监督我们的活动，并确保癌症中心成员及时获得信息并支付合理的价格。所有的 我们的活动是数据密集型的，我们与IT和生物信息学资源密切互动，以满足 从项目开始到数据的最终交付，我们都能满足用户的需求。自上次竞争性拨款以来 续签后，来自所有10个项目的130多名癌症中心成员已经使用了 GASR和癌症中心的项目约占GASR所有活动的三分之二。 对GASR的机构支持一直是例外的，包括为新的工具和 有专人来管理他们。这一资源对癌症中心的价值突出表现在大量的 基于我们设施产生的数据的高质量、同行评议的出版物。

The Gene Analysis Shared Resource (GASR) provides Mayo Clinic cancer researchers efficient, high-quality, cost-effective access to state-of-the-art genomics technologies for studying gene structure and function. Since 2003, the GASR components have been working in a coordinated fashion to provide technical expertise, instrumentation, and coordinated support for nucleic acid sequencing, gene expression profiling, and genotyping. Support for NextGen sequencing and gene expression regulation through RNAi analysis were added in 2007. The services provided by the facility include deep sequencing studies of RNA and DNA on Illumina HiSeq and MiSeq instruments, and a Pacific Biosciences Single Molecule Real Time Sequencer. In addition, we offer full-service sequencing using ABI 3730x1 sequencers, plus comprehensive genotyping and gene transcript analysis using Affymetrix Genechip™ technology and Illumina BeadChips. Methylation analyses are offered using Illumina methylation arrays and reduced representational bisulfite sequencing on Illumina HiSeq instruments. An important additional capability is offered by the RNA Interference Shared Resource, which offers special contract pricing on packaging reagents for lentiviral production and access to the complete Open Biosystems pGIPZ knockdown library to all Mayo investigators. Each of the four activities is directed by a senior, staff-level Co-Director with expertise in the area of focus. With significant institutional support, we have continued to add new instruments, personnel and services in support of Cancer Center activities. There is a well-developed cooperative administrative structure that oversees our activities and ensures timely access and affordable pricing to cancer center members. All of our activities are data intensive, and we interact closely with the IT and bioinformatics resources to meet the needs of our users, from project inception through the final delivery of data. Since the last competitive grant renewal, more than 130 Cancer Center members from all 10 programs have used the services offered by the GASR and Cancer Center projects account for about two-thirds of all the activity in the GASR. Institutional support for GASR has been exceptional, including funding for new instruments and the personnel to run them. The value of this resource to the Cancer Center is highlighted by the large number of high-quality, peer-reviewed publications that were based upon data produced in our facilities.