Sable Systems Promethion for Mouse Metabolic Analysis

项目总结/摘要 肥胖是一个主要的健康问题，彭宁顿生物医学研究中心是在最前沿的， 努力了解肥胖的原因，并开发治疗方法来对抗代谢疾病。为了 为了有效地完成这项研究使命，彭宁顿在很大程度上依赖于对小鼠肥胖模型的研究 使用研究核心基础设施，通过同时进行的能源平衡的具体评估， 测量食物摄入、能量消耗、底物氧化和身体活动。我们目前有 16个NIH资助的奖项在某种程度上依赖于这种设备。为了延续这一传统， 卓越，这个S10应用程序旨在升级和扩大我们的能力，以支持代谢研究， 申请资金购买一个32笼的Sable Promethion代谢/间接量热室系统。 我们目前的代谢室已达到最大容量，需要很长的等待时间，而较旧的Oxymax系统 功能有限，实际上超出了其预期寿命。研究生产力目前有限 因为需求超过了我们代谢室的可用性和能力，我们老化的基础设施， 增加了最终设备故障将严重损害执行资助的能力的风险 研究表明，我们老化的Oxymax系统对动物的压力更大，对许多动物来说功能不足。 实验目标。购买Sable Promethion系统，以取代使用超过10年的Oxymax 将扩大容量，升级老化的基础设施，并允许目前不可能的新科学， 我们目前的系统。

Project Summary/Abstract Obesity represents a major health issue, and Pennington Biomedical Research Center is at the forefront of the fight to both understand the causes of obesity and develop treatments to combat metabolic disease. In order to effectively purse this research mission, Pennington has relied heavily on the study of mouse models of obesity using research core infrastructure that allows the specific assessment of energy balance via the simultaneous measurement of food intake, energy expenditure, substrate oxidation and physical activity. We currently have 16 funded NIH awards that depend on this equipment in some fashion. In order to continue this tradition of excellence, this S10 application seeks to upgrade and expand our capacity to support metabolic research by requesting funds to purchase a 32 cage Sable Promethion metabolic/indirect calorimetry chamber system. Our current metabolic chambers are at max capacity, require long wait times, and the older Oxymax system is functionally limited and practically beyond its life-expectancy. Research productivity is currently limited because demand outstrips the availability and capability of our metabolic chambers, our aging infrastructure increases the risk that eventual equipment failure will severely compromise the ability to execute funded research, our aging Oxymax system is both more stressful to the animals and functionally insufficient for many experimental goals. The purchase of a Sable Promethion system to replace the over 10 year old Oxymax would expand capacity, upgrade aging infrastructure, and allow novel science that is not currently possible with our current systems.