Iron and Heme Core (Core B)

项目摘要 铁和血红素疾病中心（CIHD）的铁和血红素核心将提供金属分析， 卟啉、卟啉前体和血红素。核心将测量八种酶的酶活性， 血红素生物合成途径。这个核心在所提供的服务中是独一无二的。分析和绝对 定量细胞、组织、全血、尿液和粪便中的许多这些化合物将变得可用， 全国实验室核心还将提供一系列试剂，包括用于表达 血红素合成中的蛋白质，对这些蛋白质有反应的抗体，以及可以 用于纯化卟啉途径中间体以用于其它实验系统。核心工作人员将主持 培训课程，以协助PI，博士后研究员和研究生履行上述服务， 包括旨在进行数据分析的会议。CIHD调查人员将获得优先访问这些 通过专职核心工作人员提供服务。铁和血红素核心的目标是成为国家资源 用于在各种实验系统中测量这些酶/化合物， 变成人类

PROJECT SUMMARY   The Iron and Heme Core of the Center for Iron and Heme Disorders (CIHD) will provide analysis of metals, porphyrins, porphyrin precursors and heme. The Core will measure enzymatic activity of the eight enzymes of the heme biosynthesis pathway. This Core is unique in the services offered. Analysis and absolute quantitation of many of these compounds in cells, tissue, whole blood, urine and feces will become available to laboratories nationally. The Core will also provide a series of reagents, including plasmids for expression of proteins in heme synthesis, antibodies reactive to these proteins, and unique strains of bacteria that can be used to purify porphyrin pathway intermediates for use in other experimental systems. Core staff will host training sessions to assist PIs, post-doctoral fellows and graduate students in performing the services above, including sessions aimed at data analysis. CIHD investigators will be provided priority access to these services through dedicated Core staff. It is the goal of the Iron and Heme Core to become a national resource for the measurement of these enzymes/compounds in a diverse range of experimental systems from bacteria to human.