Cell_Tissue_Microscopy_Core

核心D -细胞和组织显微镜核心 Jyoti Jaiswal博士 核心导向器 遗传医学研究中心和神经科学研究中心研究员， 全国儿童保健系统 Anastas Popratiloff，医学博士，哲学博士 核心联合主任 首席科学家和主任，GW纳米纤维和成像中心 乔治华盛顿大学医学与健康科学学院 Shivaprasad Bhuvanendran，ME 核管理器 科学家II 遗传医学研究中心和神经科学研究中心 全国儿童保健系统 克莉丝汀布兰特纳博士 高级研究科学家，电子显微镜 GW纳米纤维和成像中心 乔治华盛顿大学医学与健康科学学院 摘要 细胞和组织显微镜核心（CTMC）的目标是提供最先进的细胞和组织 向DC-IDDRC调查人员提供成像技术，以支持、促进和加强针对IDD的 对细胞、组织和动物进行显微镜检查的研究。通过这种支持，CTMC 使DC地区的IDD研究人员能够追踪和研究各种儿科疾病， 神经发育障碍、胎儿和新生儿脑损伤和遗传疾病。CTMC已 传统上提供光学显微镜、电子显微镜和共聚焦显微镜服务。但由于 为了满足DC-IDD研究人员对增强成像分辨率日益增长的需求，Core获得了新的 仪器，允许扩展其服务，包括先进的电子显微镜（EM）技术， 多光子和超分辨率成像能力。这些发展将使成像从纳米级到 整个组织和胚胎鳞片。这一增长得到了核心业务显著扩张的补充。 基础设施，允许处理大型数据集，并对大型结构和功能进行分析 数据集。核心项目的进一步发展将大大提高缺碘症研究人员的能力， 结合光遗传学和电生理学进行体内和体外功能成像。支持 在CTMC的这一增长，两个新的核心经理已被雇用，谁带来了他们的专业知识在体内 功能成像、EM、相关光和EM成像。这些人员和其他专职核心工作人员 他们都是实践研究人员，不仅通过指定的核心活动提供他们的专业知识， 也通过合作项目。核心的这些能力得到了合作的进一步补充， 在核心和多家显微镜和成像技术公司之间建立。此外，核心 与其他DC-IDDRC核心接口，包括神经行为评估，基因组学和蛋白质组学， 以及人类和动物成像核心。我们将利用CTMC工作人员提供的专业知识和服务， 利用与此核心相关的用户的参与，并与显微镜建立合作伙伴关系 公司，以提供以下服务：1）培训研究人员在显微镜成像 方法，2）帮助研究人员正确选择和使用光和EM工具来检查细胞 3）帮助开发和实施对所获得的数据进行定量分析的方法， 每个项目，4）保持高质量的数据采集，分析和出版，以及5）培训和 教育下一代碘缺乏病研究人员应用显微镜技术， 实际操作培训、特定技术教育和研讨会。 !

CORE D – CELL AND TISSUE MICROSCOPY CORE Jyoti Jaiswal, PhD Core Director Investigator, Center for Genetic Medicine Research & Center for Neuroscience Research, Children's National Health System Anastas Popratiloff, MD, PhD Core Co-Director Lead Scientist and Director, GW Nanofabrication and Imaging Center The George Washington University School of Medicine and Health Sciences Shivaprasad Bhuvanendran, ME Core Manager Staff Scientist ll Center for Genetic Medicine Research & Center for Neuroscience Research Children’s National Health System Christine Brantner, PhD Senior Research Scientist, Electron Microscopy GW Nanofabrication and Imaging Center George Washington University School of Medicine and Health Sciences Abstract The goal of the Cell and Tissue Microscopy Core (CTMC) is to provide access to state-of-the-art cell and tissue imaging technologies to DC-IDDRC investigators, in order to support, facilitate and enhance IDD-targeted research that involves microscopic examination of cells, tissues and animals. Through this support, the CTMC enables IDD researchers in the DC region to pursue and investigate a variety of pediatric diseases with a focus on neurodevelopmental disorders, fetal and neonatal brain injury, and genetic diseases. The CTMC has traditionally provided light microscopy, electron microscopy and confocal microscopy services. However, due to the growing need of DC-IDD researchers for enhanced imaging resolution, the Core has acquired new instruments that allow expansion of its services to include advanced electron microscopy (EM) technologies, Multiphoton, and super resolution imaging abilities. These developments will enable imaging from nanoscale to whole tissue and embryo scale. This growth has been supplemented by the significant expansion in Core’s infrastructure, which allow to handle large datasets, and carry out analysis of large structural and functional data sets. Additional developments in the Core will significantly enhance the ability of IDD researchers to perform in vivo and ex vivo functional imaging with combined optogenetics and electrophysiology. To support this growth in the CTMC, two new Core managers have been hired, who bring their expertise in in vivo functional imaging, EM, and correlative light and EM imaging. These and other dedicated Core staff members are all practicing researchers and offer their specific expertise not only through designated Core activities, but also through collaborative projects. These abilities of the Core are further supplemented by the collaborations forged between the Core and multiple microscopy and imaging technology companies. Additionally, the Core interfaces with other DC-IDDRC Cores, including the Neurobehavioral Evaluation, Genomics and Proteomics, and Human and Animal Imaging Cores. We will use the expertise and services offered by the CTMC staff, leverage on the involvement of users affiliated with this Core, and create partnerships with microscopy companies, in order to provide the following services: 1) training researchers in microscopy-based imaging approaches, 2) assisting researchers in proper choice and use of light and EM tools for examination of cells and tissues, 3) helping develop and implement approaches for quantitative analysis of the data obtained for each project, 4) maintaining high quality of data acquisition, analysis and publication, and 5) training and educating the next generation of IDD researchers in the application of microscopy-based technologies through hands on training, education on specific technologies, and workshops. !