A High-Throughput Molecular Profiling Core for the CaST Center

项目总结 分子图谱核心将提供集成的药物筛选和高通量表达图谱， 为中心的每个项目提供单细胞RNA-Seq、基本RNA-Seq和外显子组测序服务。vbl.使用 我们最近开发的平板序列技术，我们可以生产高维表情图谱 高通量药物筛查中的每一口井。我们还将提供平板序列的微流体实现 用于中心研究人员将用来鉴定表型的大规模单细胞表达谱 引起耐药性的肿瘤细胞亚群。中心调查人员还需要基本的 包括深层外显子组和RNA-Seq的测序服务，以识别突变，以便在 单细胞水平和基因融合。核心将提供量身定制和具有成本效益的图书馆准备和 测序服务，以促进这些努力。

PROJECT SUMMARY The Molecular Profiling Core will provide integrated drug screening and high-throughput expression profiling, single cell RNA-Seq, and basic RNA-Seq and exome sequencing services to every project in the center. Using our recently developed PLATE-Seq technology, we can produce high-dimensional expression profiles across every well in a high-throughput drug screen. We will also provide a microfluidic implementation of PLATE-Seq for large-scale single cell expression profiling which will be used by center investigators to identify phenotypic subpopulations of tumor cells that give rise to drug resistance. Center investigators also require basic sequencing services including deep exome and RNA-Seq to identify mutations for targeted profiling at the single cell level and gene fusions. The Core will provide tailored and cost effective library preparation and sequencing services to facilitate these efforts.