Flow Cytometry and Cell Sorting Facility

项目摘要 流式细胞术和细胞分选设备由Abramson癌症中心于1980年建立， 自1981年以来，NCI癌症中心支持补助金一直在批准和资助。在过去 竞争性续约后，该共享资源获得了“特殊”的价值评估。该设施是 被公认为最大和最全面的学术流式细胞术共享资源之一， 美国，提供广泛的分析和分选细胞仪，专家咨询， 广泛的教育计划。Jonni摩尔，博士，病理学和实验室医学教授， 自1990年以来，该设施。她拥有免疫学博士学位，是世界著名的免疫学和免疫学专家。 高维流式细胞术的应用和共享资源操作。技术人员是 由MCI的Charles Pletcher管理，他自1987年以来一直在该设施工作。他带来了超过25年的 广泛的技术仪器和应用经验的设施。该设施的工作人员是 由经验丰富的技术专家组成，每个人平均拥有12年的经验，他们提供ACC 成员获得尖端的细胞技术，以及在实验设计和 数据评价在本项目期间，流式细胞术和细胞分选设施仍然是一个 最常用的CCSG资助的阿布拉姆森癌症中心共享资源，并继续 体验成长ACC成员占使用该设施的265名研究人员中的110名（41%） 在本报告所述期间（2013年10月1日至2014年9月30日），其中绝大多数（102/110）接受了同行审评 经费CCSG的支持占拟议基金预算的10%，其余资金来自 从收费，额外的赠款支持和机构支持。共享资源的成功 在项目期间，通过增加仪器、招聘工作人员、扩大 培训计划的增加以及ACC成员对细胞学的兴趣增加。

Project Summary The Flow Cytometry and Cell Sorting Facility was established by the Abramson Cancer Center in 1980, and has been continuously approved and funded by the NCI Cancer Center Support Grant since 1981. In the last competitive renewal, this Shared Resource received an “exceptional” merit evaluation. The Facility is recognized as one of the largest and most comprehensive academic flow cytometry shared resources in the United States, offering access to a broad range of analytical and sorting cytometers, expert consultation, and an extensive educational program. Jonni Moore, PhD, Professor of Pathology and Laboratory Medicine, has directed the Facility since 1990. She has a doctorate in immunology and is a noted worldwide expert in both the application of high dimensional flow cytometry and shared resource operation. The technical staff is managed by Charles Pletcher, MCI, who has been with the Facility since 1987. He brings over 25 years of extensive technical instrumentation and application experience to the Facility. The staff of the Facility is comprised of experienced technologists, each with an average of 12 years experience, who offer ACC members access to cutting edge cytometric technology, as well as expert support in experimental design and data evaluation. During the current project period, the Flow Cytometry and Cell Sorting Facility remained one of the most frequently used CCSG-funded Shared Resources of the Abramson Cancer Center, and continued to experience growth. ACC members accounted for 110 of the 265 researchers (41%) who used the Facility during the reporting period (10/01/13-09/30/14), the vast majority of whom (102/110) have peer-reviewed funding. CCSG support represents 10% of the proposed Facility budget with the remaining funding coming from charge backs, additional grant support and Institutional support. The success of the Shared Resource during the project period has been facilitated by the addition of instrumentation, recruitment of staff, expansion of training programs and the increased interest in cytomics within the ACC membership.