Shared Resource: Cytometry and Cell Sorting

核心：细胞学和细胞分选共享资源(细胞和体内生物学组) 项目总结 流式细胞术是癌症研究中一项基本的和广泛应用的技术，以帮助阐明 肿瘤抑制和癌基因、细胞周期进展、转化病毒的机制及其评价 癌症疗法。它也是癌症干细胞、血管生成、炎症、转录的研究不可或缺的一部分。 肿瘤细胞的调控和DNA断裂与修复的机制。细胞计量学和细胞学的目标 分类共享资源(CCSSR)是为广泛的癌症中心研究人员提供访问 具有成本效益的最先进的仪器、专业知识和培训，以满足他们的细胞分选和分析需求。 这项技术继续快速发展，特别是随着新工具和试剂的出现， 增加计算能力和更具成本效益的光学设备。与时俱进 技术和用户增长在过去的4年里，CCSSR通过新的实验室扩大了空间 翻新，在我们附属的德克萨斯儿童医院癌症中心增加了一个卫星设施，并获得了 对以前的现有设备进行了大幅升级，并购置了更多新的主要设备。这个 主要设施位于中央位置，训练有素的用户可全天24小时使用 每周通过钥匙卡访问。目前的主要仪器包括四个完全荧光激活的细胞分选器， 六台流动分析仪以及基于磁性和微流体的细胞分选器。《资源》由克里斯汀博士指导 Beeton和Joel Sederstrom先生，他们分别在流式细胞仪方面拥有超过10年和15年的经验， 并配备三名全职有经验的流式细胞仪操作员，他们进行操作员辅助的细胞术，以及 协助用户进行数据分析。为了确保服务的最佳使用，CCSSR提供咨询，个别- 量身定制的培训，以及样品制备、流动分析和细胞分选的方案。这是一款使用量很大的 去年为125名癌症中心研究人员提供服务的共享资源， 与CCSG奖第一年的83名用户相比，有所增加。未来的计划包括增加新技术 以及用于基于图像和基于质量的细胞分析的仪器，这些仪器正在突破传统的 流式细胞术和进一步扩大空间和研究人员需要这些新技术和 预计CCSSR将继续增长。

CORE: Cytometry and Cell Sorting Shared Resource (Cell and in vivo Biology Group) PROJECT SUMMARY Flow cytometry is an essential and broadly applicable technology for cancer research to assist with elucidating mechanisms of tumor suppressor and oncogenes, cell-cycle progression, transforming viruses, and to evaluate cancer therapies. It is also integral to studies of cancer stem cells, angiogenesis, inflammation, transcriptional regulation in tumor cells and mechanisms of DNA break and repair. The goal of the Cytometry and Cell Sorting Shared Resource (CCSSR) is to provide a wide range of cancer center investigators with access to cost-effective state-of-the-art instrumentation, expertise and training for their cell sorting and analysis needs. This technology continues to develop at a rapid pace, especially with the advent of novel tools and reagents, increased computational capacity and more cost-effective optical equipment. To keep pace with changing technologies and growth of users over the past 4 years, the CCSSR has expanded its space with new lab renovations, added a satellite facility at our affiliated Texas Childrens Hospital Cancer Center and acquired substantial upgrades of previously exsiting equipment and acquired additional new major equipment. The main facility is housed in centrally located space and is available to trained users 24 hours a day, seven days a week by key-card access. Current major instrumentation includes four fully florescence-activated cell sorters, six flow analyzers and magnetic- and microfluidic-based cell sorters. The Resource is directed by Dr. Christine Beeton and Mr. Joel Sederstrom, who have over ten and 15 years of experience in flow cytometry respectively, and is staffed by three full-time experienced flow cytometrists who perform operator-assisted cytometry, and assist users with data analysis. To ensure optimal use of services, CCSSR provides consultations, individually- tailored training, and protocols for sample preparation, flow analysis and cell sorting. This is a heavily used Shared Resource that provided service for 125 Cancer Center investigators during the last year, a substantial increase from 83 users in the first year of the CCSG award. Future plans include addition of new technologies and instrumentation for image- and mass-based cytometry that are pushing the boundaries of conventional flow cytometry and further expansion of space and research staff required for these new technologies and anticipated continued growth of CCSSR.