Shared Resource: Metabolomics

核心：代谢组学共享资源(Omics Group) 项目总结 长期以来，癌症的发生和发展一直被认为是多因素的。开始理解 癌症进展从系统的角度来看，我们需要表征和整合各种分子 分析了各种生物样品中涉及的成分，定义了系统生物学的新时代，或 经济学。-组学时代的出现已经将重点从个体基因和 蛋白质来检测表达的基因组和蛋白质组的一个重要组成部分。破译 区分进展为晚期疾病的肿瘤亚群的分子网络将描绘出 癌症的病因学，以及有助于识别患者的生物标记物的识别 这应该得到治疗。尽管这样的研究是使用全球转录组学进行的，而且对一些人来说 广泛的蛋白质组学平台，在研究全球代谢领域取得的成果要少得多 改装。目前的应用旨在创建一个以癌症为中心的代谢组学共享资源，以提供 服务、基础设施、专业知识、支持和培训，以测量广泛的 使用各种正交技术平台的样本，这些平台一起可以测量显著的广度 代谢物。具体地说，这一共享资源将衡量极地、中极和中极的定性和定量水平 包括脂质在内的极性和非极性代谢物以及测量细胞系、组织、 血浆、血清和尿液。

CORE: Metabolomics Shared Resource (Omics Group) PROJECT SUMMARY Development and progression of cancer has long been known to be multifactorial. To begin to understand cancer progression with a systems perspective, we need to characterize and integrate the various molecular components involved across the various biospecimens analyzed, defining the new era of Systems Biology, or OMICS. The emergence of the “-omics” era has shifted the focus from the assessment of individual genes and proteins to examination of a substantial component of the expressed genome and proteome. Deciphering the molecular networks that distinguish subsets of tumors that progress to advanced disease will delineate the etiology of cancer, as well as lead to the identification of biomarkers that will aid in the identification of patients that should be treated. Although such studies have been carried out using global transcriptomics, and to some extent proteomics platforms, much less has been achieved in the domain of studying global metabolic alterations. The current application aims to create a cancer-centric metabolomics shared resource to provide service, infrastructure, expertise, support and training on measurement of metabolite levels in a broad range of specimens using various orthogonal technology platforms that together can measure a significant breadth of the metabolome. Specifically, this shared resource will measure qualitative and quantitative levels of polar, mid polar and non-polar metabolites including lipids as well as measure pathway activity in cell lines, tissues, plasma, serum and urine.