Acquisition of a high-performance liquid chromatography system

项目总结(1R01GM114080行政副刊) 作为我目前项目(R01GM114081)的一部分，我们需要合成多肽，将它们与聚合物结合， 提纯偶联物，并对纳米颗粒进行表征。目前，我们正在完成 目标2并进行目标3的实验。然而，为了以期望的速度进行研究， 我们需要合成聚合物、多肽和偶联物，提纯和表征它们。此外，我们 还需要凝胶渗透色谱装置来表征合成的聚合物和聚合物- 多肽结合物。 我们在实验室使用高效液相系统来提纯合成的多肽。我们正在使用高效液相色谱仪 凝胶渗透柱体系测定聚合物的相对分子质量和多分散性指数 聚合物及其多肽结合物。这乐器已有十多年的历史了。由于年代久远， 仪器经常会出现维护问题和维修。高效液相色谱的问题正在拖慢我们在 项目，我正在积极寻找资金来源，以取代旧的高效液相仪器。 岛津的一台带有荧光和折射率探测器的新高效液相色谱仪售价为64,145美元 (请求中包含的报价)。由于我们目前的高效液相系统来自岛津，我联系了他们以获得 一种新乐器的报价。我们熟悉仪器、控制、软件和故障排除 岛津高效液相色谱系统的一些方面。申请的资金将允许我们购买一台新的岛津 高效液相-凝胶渗透色谱联用系统及时完成资助项目。该乐器还将有助于 NIH R01奖项的竞争性续签。

Project Summary (1R01GM114080 Administrative Supplement) As a part of my current project (R01GM114081), we need to synthesize peptides, conjugate them with polymers, purify the conjugates, and characterize the nanoparticles. Currently, we are completing the studies proposed in the Aim 2 and proceeding to the experiments of Aim 3. However, for the studies to proceed at the desired pace, we need to synthesize the polymers, peptides, the conjugates, purify and characterize them. In addition, we also need a gel-permeation chromatography set-up to characterize the synthesized polymers and the polymer- peptide conjugates. We are using an HPLC system in our laboratory for purifying the synthesized peptides. We are using the HPLC system with a gel-permeation column for determining the molecular weights and the polydispersity indices of the polymers and their peptide conjugates. The instrument is more than ten years old. Due to the age, the instrument is subject to frequent maintenance issues and servicing. The HPLC problems are slowing us on the project, and I am actively looking into funding sources to replace the old HPLC instrument. A new HPLC instrument with fluorescence and refractive index detectors would cost $64,145 from Shimadzu (quotation included in the request). Since our current HPLC system is from Shimadzu, I contacted them for the quotation of a new instrument. We are familiar with the instrument, controls, software, and troubleshooting aspects of the Shimadzu HPLC system. The requested funds would allow us to purchase a new Shimadzu HPLC-GPC combined system for timely-conclusion of the funded project. The instrument would also aid in the competitive renewal of the NIH R01 award.