Core Grant for Vision Research

摘要 我们中心核心的总体目标是提供专业知识、设施和设备，以促进和扩大 威斯康星大学麦迪逊分校的视觉研究。这将由四个核心来完成。向量 基因传递/定量分子生物学核心将促进基因传递方法的使用，即qPCR 以及核心用户使用的其他分子方法。病理和成像核心将促进共焦的使用 显微镜、荧光显微镜和光学显微镜、组织病理学、专门的组织染色方法和图像 按核心用户分析。动物模型和器官培养核心将促进非人类的使用 灵长类、啮齿动物等动物眼部疾病模型的建立，利用OCT、ERG、VEP来评估视力 功能，以及使用其他视觉结构和功能结果的衡量标准。生物统计学和 流行病学资源核心将促进适当的研究设计、权力计算的使用、适当的使用 重要的统计指标，以及针对核心用户的适当数据分析方法。

SUMMARY The overall goal of our Center Core is to provide expertise, facilities, and equipment to foster and expand vision research at the University of Wisconsin-Madison. This will be accomplished by four Cores. The Vector and Gene Delivery/Quantitative Molecular Biology Core will facilitate the use of gene delivery methods, qPCR and other molecular methods by core users. The Pathology and Imaging Core will facilitate the use of confocal microscopy, fluorescent and light microscopy, histopathology, specialized tissue staining methods, and image analysis by core users. The Animal Models and Organ Culture Core will facilitate the use of non-human primate, rodent and other animal models of ocular disease, the use of OCT, ERG, VEP to assess visual function, and the use of other visual structure and function outcome measures. The Biostatistics and Epidemiology Resource Core will facilitate proper study designs, the use of power calculations, proper use of statistical measures of significance, and proper data analysis methods for Core users.