Core Grant for Vision Research

威斯康星大学视觉研究人员P30核心助学金 提出三个单元：基因传递/量子分子生物学；病理学和成像；以及动物 模特与眼器官文化。高效地将基因输送到细胞的能力是一种有效的新疗法 它是治疗眼科疾病的一种有效方法，是一种必不可少的研究工具。基因传递模块将 构建载体，准备这些材料的高滴度库存供研究人员使用，并协助调查人员 用基因传递的方法。定量方法在分子生物学中的应用越来越广泛。特别是， 逆转录聚合酶链式反应定量基因表达及基因表达变化的阵列分析 技术基因传递/定量分子生物学模块将提供专门的技术 这些领域的专门知识，并将增强目前使用这些技术的研究人员的能力 技术将它们添加到他们的研究计划中。病理分析和复杂的成像 随着对翻译研究的重视，方法变得越来越重要。这个 病理和成像模块将提供先进的组织学、显微镜和图像分析，以及 组织切片和培养细胞的形态计量学。新疗法的开发和改善我们的 对视觉系统功能的理解依赖于将体外所做的工作转移到动物模型上。这个 动物模型和眼器官培养模块将帮助研究人员使用动物模型(灵长类，猫， 兔和啮齿动物)，实验性青光眼诱导，成像和眼生理学。这方面的专业知识将有助于 核心用户在帮助其感染剂、药物、操作和治疗效果方面的努力 啮齿动物、猫、兔和灵长类动物眼功能的载体/结构。 在上一个供资期间，总共援助了28笔符合条件的NEI赠款。截至提交之日 到目前为止，已有188篇同行评审的论文涉及核心的使用。

This P30 Core Grant for Vision Research application from the University of Wisconsin Vision Researchers proposes three modules; Gene Delivery/Quantitafive Molecular Biology; Pathology and Imaging; and Animal Model and Eye Organ Culture. The ability to efficiently deliver genes to cells is a powerful new therapeutic approach in treating ocular disease and is an essential research tool. The Gene delivery module will construct vectors, prepare high titer stocks of these materials for use by researchers, and assist investigators with gene delivery methods. The use of quantitative methods in molecular biology is growing. In particular, the quantitation of gene expression by RT-PCR and the analysis of gene expression changes using array technology. The Gene Delivery/Quantitative Molecular Biology Module will provide dedicated technical expertise to these areas and will enhance the abilities of those researchers currently using these technologies to add them to their research programs. Pathology analysis and sophisticated imaging methods have become increasingly important, as the emphasis on translational research has grown. The Pathology and Imaging Module will provide advanced histology, microscopy and image analysis, and morphometry of tissue sections and cultured cells. The development of new therapies and improving our understanding of visual system function relies on transferring work done in vitro to animal models. The Animal Model and Eye Organ Culture Module will assist investigators in using animal models (primate, cat, rabbit and rodent), experimental glaucoma induction, imaging and ocular physiology. This expertise will aid the efforts of core users in assisting the effects of their infectious agents, drugs, manipulations, and vectors/constructs on ocular function in rodents, cats, rabbits and primates. During the previous funding period a total of 28 qualifying NEI grants were assisted. As of the submission date, 188 peer reviewed papers have involved use of the core.