Flow Cytometry & Cell Sorter Core

核心设施的开发是INBRE的PI的一个学习经验。INBRE的主要教训是，在一个人口少、土地大、调查人员有限的国家，必须开发核心设施，提供无法通过商业实体购买的服务。这当然适用于流动和细胞分选，因为在整个北达科他州没有其他能够分选细胞的设施。同样，通过商业实体或远离家庭实验室的学术中心进行流动是非常困难的，如果不是不可能的话。对于细胞分选尤其如此，在大多数情况下，需要立即处理和/或培养产品。这种情况的一个主要例子是干细胞的尖端技术，如果没有能力对所产生的细胞进行分类和捕获以进行培养和/或分析，那么该领域的研究即使不是不可能，也是很困难的。INBRE已经确定了这一核心是必不可少的研究，教学和服务的进一步发展在NO。简单地说，在他们本科生涯的某个时刻，每个注定要进入研究生或健康专业管道的本科生都应该接触过流动和细胞分选。同样的道理也适用于那些想要获得博士后奖学金的研究生和想要成为住院医生的医科学生。

The development of the core facilities has been a learning experience for the PI of the NO INBRE. The major lesson learned since initial funding of the NO INBRE is that in a low population, large land mass state with a limited investigator base, one must develop core facilities that provide services that cannot be purchased through commercial entities. This certainly applies to flow and cell sorting since there is no other facility capable of sorting cells in the entire state of North Dakota. Likewise, it is very difficult, if not impossible, to perform flow through commercial entities or at academic centers distant from one's home laboratory. This is especially true for the sorting of cells, where in most instances immediate processing and/or culture of the product is necessary. A prime example of this situation is in the cutting-edge technology of stem cells, where research in this area is difficult, if not impossible, without the ability to sort and capture the resulting cells for culture and/or analysis. The NO INBRE has identified this core as essential for the further development of research, teaching and service in NO. Simply stated, at some juncture in their undergraduate career, every undergraduate student destined to enter the graduate student or health professional pipeline should have been exposed to flow and cell sorting. The same is true for graduate students destined for postdoctoral fellowships and medical students progressing to residencies.